Detailed information of TA system TA107907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2960580..2961230
• Replicon: chromosome
• Accession: NZ_CP030768
• Organism: Escherichia coli strain 2017C-4173W12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DSB71_RS15030
• Protein ID: WP_000263532.1
• Coordinates: 2960580..2960921 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: DSB71_RS15035
• Protein ID: WP_000212552.1
• Coordinates: 2960931..2961230 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSB71_RS15005	2955615..2956715	+	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-
DSB71_RS15010	2956715..2957014	+	300	WP_000867281.1	rod-binding protein	-
DSB71_RS15015	2957190..2958566	+	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
DSB71_RS15020	2958581..2959510	+	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
DSB71_RS15025	2959527..2960504	+	978	WP_001195938.1	hypothetical protein	-
DSB71_RS15030	2960580..2960921	+	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DSB71_RS15035	2960931..2961230	+	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
DSB71_RS15040	2961288..2962130	-	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
DSB71_RS15045	2962616..2963530	+	915	WP_000949083.1	lateral flagellin LafA	-
DSB71_RS15055	2963735..2965051	+	1317	WP_000609678.1	flagellar filament capping protein FliD	-
DSB71_RS15060	2965074..2965466	+	393	WP_000285322.1	flagellar export chaperone FliS	-
DSB71_RS15065	2965471..2965782	+	312	WP_000415004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T107907 WP_000263532.1 NZ_CP030768:2960580-2960921 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T107907 NZ_CP030768:2960580-2960921 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT107907 WP_000212552.1 NZ_CP030768:2960931-2961230 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT107907 NZ_CP030768:2960931-2961230 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGTGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7