Detailed information of TA system TA107897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1199492..1200291
• Replicon: chromosome
• Accession: NZ_CP030768
• Organism: Escherichia coli strain 2017C-4173W12

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: B7NDB6
• Locus tag: DSB71_RS06075
• Protein ID: WP_000347269.1
• Coordinates: 1199827..1200291 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0YUB5
• Locus tag: DSB71_RS06070
• Protein ID: WP_001309780.1
• Coordinates: 1199492..1199827 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSB71_RS06055	1195277..1196047	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
DSB71_RS06060	1196063..1197397	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
DSB71_RS06065	1197772..1199343	+	1572	WP_001273738.1	galactarate dehydratase	-
DSB71_RS06070	1199492..1199827	+	336	WP_001309780.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
DSB71_RS06075	1199827..1200291	+	465	WP_000347269.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
DSB71_RS06080	1200346..1201155	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
DSB71_RS06090	1201404..1202684	+	1281	WP_000681943.1	tagatose-bisphosphate aldolase subunit KbaZ	-
DSB71_RS06095	1202707..1203180	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
DSB71_RS06100	1203191..1203970	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
DSB71_RS06105	1203960..1204838	+	879	WP_113621712.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
DSB71_RS06110	1204856..1205290	+	435	WP_000948818.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1190250..1200291	10041

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T107897 WP_000347269.1 NZ_CP030768:1199827-1200291 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Nucleotide

• Length: 465 bp

>T107897 NZ_CP030768:1199827-1200291 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCTTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGGCAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12345.94 Da
• Isoelectric Point: 4.8616

>AT107897 WP_001309780.1 NZ_CP030768:1199492-1199827 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT107897 NZ_CP030768:1199492-1199827 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACACC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829IY86

Antitoxin

• AlphaFold DB: V0YUB5