Detailed information of TA system TA107891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 5341960..5342654
• Replicon: chromosome
• Accession: NZ_CP030767
• Organism: Escherichia coli strain 2017C-4109

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: DSB73_RS28360
• Protein ID: WP_001263495.1
• Coordinates: 5342256..5342654 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: DSB73_RS28355
• Protein ID: WP_000554757.1
• Coordinates: 5341960..5342253 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSB73_RS28335	5337671..5338168	+	498	Protein_5111	REP-associated tyrosine transposase RayT	-
DSB73_RS28340	5338313..5340025	-	1713	Protein_5112	flagellar biosynthesis protein FlhA	-
DSB73_RS28345	5339997..5340782	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
DSB73_RS28350	5340853..5341908	+	1056	WP_001226186.1	DNA polymerase IV	-
DSB73_RS28355	5341960..5342253	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DSB73_RS28360	5342256..5342654	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DSB73_RS28365	5342664..5343116	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
DSB73_RS28370	5343435..5343638	+	204	Protein_5118	RNA ligase RtcB family protein	-
DSB73_RS28375	5343637..5344158	+	522	Protein_5119	peptide chain release factor H	-
DSB73_RS28380	5344215..5345672	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
DSB73_RS28385	5345933..5346391	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	5346987..5347067	+	81	NuclAT_11	-	-
-	5346987..5347067	+	81	NuclAT_11	-	-
-	5346987..5347067	+	81	NuclAT_11	-	-
-	5346987..5347067	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T107891 WP_001263495.1 NZ_CP030767:5342256-5342654 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T107891 NZ_CP030767:5342256-5342654 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT107891 WP_000554757.1 NZ_CP030767:5341960-5342253 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT107891 NZ_CP030767:5341960-5342253 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6