Detailed information of TA system TA107842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 122318..122969
• Replicon: plasmid unnamed1
• Accession: NZ_CP030755
• Organism: Acinetobacter schindleri strain H3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9FYW4
• Locus tag: DPR03_RS14985
• Protein ID: WP_000269911.1
• Coordinates: 122318..122674 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DPR03_RS14990
• Protein ID: WP_001140620.1
• Coordinates: 122667..122969 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPR03_RS14960	118368..119036	-	669	Protein_125	IS3-like element ISAba22 family transposase	-
DPR03_RS14965	119106..120196	+	1091	WP_110925086.1	IS4-like element ISAba1 family transposase	-
DPR03_RS14970	120158..120577	-	420	WP_071321254.1	hypothetical protein	-
DPR03_RS14975	120694..121101	+	408	WP_071321253.1	hypothetical protein	-
DPR03_RS14980	121115..121441	+	327	WP_005098935.1	hypothetical protein	-
DPR03_RS14985	122318..122674	+	357	WP_000269911.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DPR03_RS14990	122667..122969	+	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
DPR03_RS14995	123011..123172	-	162	Protein_132	IS6 family transposase	-
DPR03_RS15000	123266..123658	-	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
DPR03_RS15005	123748..125028	+	1281	WP_005093338.1	cation transporter	-
DPR03_RS15010	125092..125301	+	210	Protein_135	IS6 family transposase	-
DPR03_RS15015	125344..125622	-	279	Protein_136	transposase	-
DPR03_RS15020	125806..126090	-	285	WP_005206902.1	antibiotic biosynthesis monooxygenase	-
DPR03_RS15025	126111..127109	-	999	WP_004682270.1	zinc-binding alcohol dehydrogenase family protein	-
DPR03_RS15030	127181..127921	-	741	Protein_139	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..223569	223569
	-	inside	IScluster/Tn	-	-	113199..125544	12345

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13600.60 Da
• Isoelectric Point: 7.1651

>T107842 WP_000269911.1 NZ_CP030755:122318-122674 [Acinetobacter schindleri]
MWTVITTDLFNQWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T107842 NZ_CP030755:122318-122674 [Acinetobacter schindleri]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAATCTACACAAGAGAAGGTCCTAGC
TGCACTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGCCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGCGTTCAACATCGCGGTAGACCTTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATCGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT107842 WP_001140620.1 NZ_CP030755:122667-122969 [Acinetobacter schindleri]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT107842 NZ_CP030755:122667-122969 [Acinetobacter schindleri]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Structures

Toxin

• AlphaFold DB: A0A6A1RIA3