Detailed information of TA system TA107823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4893771..4894366
• Replicon: chromosome
• Accession: NZ_CP030351
• Organism: Pseudomonas aeruginosa strain AR_460

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CSC46_RS23110
• Protein ID: WP_003113526.1
• Coordinates: 4893771..4894049 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC46_RS23115
• Protein ID: WP_003113527.1
• Coordinates: 4894061..4894366 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC46_RS23090	4889197..4890435	+	1239	WP_003162396.1	C69 family dipeptidase	-
CSC46_RS23095	4890497..4891144	+	648	WP_003095021.1	carbonate dehydratase	-
CSC46_RS23100	4891214..4893442	-	2229	WP_003146080.1	TonB-dependent receptor	-
CSC46_RS23110	4893771..4894049	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC46_RS23115	4894061..4894366	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
CSC46_RS23125	4894772..4895872	-	1101	WP_112208180.1	redox-regulated ATPase YchF	-
CSC46_RS23130	4895913..4896497	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CSC46_RS23135	4896539..4897153	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
CSC46_RS23140	4897270..4898211	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CSC46_RS23150	4898378..4899226	-	849	WP_003117426.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T107823 WP_003113526.1 NZ_CP030351:4893771-4894049 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T107823 NZ_CP030351:4893771-4894049 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT107823 WP_003113527.1 NZ_CP030351:4894061-4894366 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT107823 NZ_CP030351:4894061-4894366 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3