Detailed information of TA system TA107808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2180139..2180814
• Replicon: chromosome
• Accession: NZ_CP030347
• Organism: Enterobacter hormaechei strain AR_038

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CSB67_RS12080
• Protein ID: WP_032619177.1
• Coordinates: 2180515..2180814 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: CSB67_RS12075
• Protein ID: WP_015571639.1
• Coordinates: 2180139..2180504 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB67_RS12055	2176595..2177326	+	732	WP_017382760.1	methyltransferase domain-containing protein	-
CSB67_RS12060	2177323..2177925	-	603	WP_017382759.1	short chain dehydrogenase	-
CSB67_RS12065	2178023..2178910	+	888	WP_032619173.1	LysR family transcriptional regulator	-
CSB67_RS12070	2178931..2180112	+	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
CSB67_RS12075	2180139..2180504	-	366	WP_015571639.1	HigA family addiction module antidote protein	Antitoxin
CSB67_RS12080	2180515..2180814	-	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSB67_RS12085	2181065..2182225	-	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
CSB67_RS12090	2182245..2183900	-	1656	Protein_2075	ATP-binding cassette domain-containing protein	-
CSB67_RS12095	2183933..2184901	+	969	WP_080288382.1	IS5-like element IS903B family transposase	-
CSB67_RS12100	2185065..2185682	+	618	WP_032634496.1	DUF1819 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2183978..2184901	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T107808 WP_032619177.1 NZ_CP030347:c2180814-2180515 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T107808 NZ_CP030347:c2180814-2180515 [Enterobacter hormaechei]
ATGATTAATCATTCTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT107808 WP_015571639.1 NZ_CP030347:c2180504-2180139 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT107808 NZ_CP030347:c2180504-2180139 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9