Detailed information of TA system TA107777

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 7528..7792
• Replicon: plasmid unnamed2
• Accession: NZ_CP030340
• Organism: Escherichia coli strain AR_451

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: CSC38_RS26900
• Protein ID: WP_024179514.1
• Coordinates: 7640..7792 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	7528..7590 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC38_RS26885	2767..5058	-	2292	WP_001289267.1	hypothetical protein	-
CSC38_RS26890	5051..6121	-	1071	WP_015059313.1	IncI1-type conjugal transfer protein TrbB	-
CSC38_RS26895	6140..7348	-	1209	WP_024171190.1	IncI1-type conjugal transfer protein TrbA	-
-	7528..7590	-	63	NuclAT_0	-	Antitoxin
-	7528..7590	-	63	NuclAT_0	-	Antitoxin
-	7528..7590	-	63	NuclAT_0	-	Antitoxin
-	7528..7590	-	63	NuclAT_0	-	Antitoxin
CSC38_RS26900	7640..7792	+	153	WP_024179514.1	Hok/Gef family protein	Toxin
CSC38_RS26905	7864..8115	-	252	WP_001291965.1	hypothetical protein	-
-	8509..8560	-	52	NuclAT_1	-	-
-	8509..8560	-	52	NuclAT_1	-	-
-	8509..8560	-	52	NuclAT_1	-	-
-	8509..8560	-	52	NuclAT_1	-	-
CSC38_RS27445	9400..9576	-	177	WP_001054898.1	hypothetical protein	-
CSC38_RS26915	9785..9994	-	210	WP_015059315.1	hemolysin expression modulator Hha	-
CSC38_RS26920	10092..10706	-	615	WP_015059316.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34927	34927

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5795.12 Da
• Isoelectric Point: 8.7948

>T107777 WP_024179514.1 NZ_CP030340:7640-7792 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T107777 NZ_CP030340:7640-7792 [Escherichia coli]
ATGCCACAGCGAACATTTTTAATGATGTTGATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGATTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT107777 NZ_CP030340:c7590-7528 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin