Detailed information of TA system TA107765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4007197..4007799
• Replicon: chromosome
• Accession: NZ_CP030337
• Organism: Escherichia coli strain AR_451

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: CSC38_RS21540
• Protein ID: WP_000897302.1
• Coordinates: 4007197..4007508 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC38_RS21545
• Protein ID: WP_000356397.1
• Coordinates: 4007509..4007799 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC38_RS21515	4003111..4003710	+	600	WP_001296610.1	glucose-1-phosphatase	-
CSC38_RS21520	4003704..4004576	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CSC38_RS21525	4004573..4005010	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CSC38_RS21530	4005055..4005996	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CSC38_RS21535	4006060..4006968	-	909	WP_001385591.1	alpha/beta hydrolase	-
CSC38_RS21540	4007197..4007508	+	312	WP_000897302.1	hypothetical protein	Toxin
CSC38_RS21545	4007509..4007799	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CSC38_RS21555	4008158..4008436	+	279	WP_001296612.1	hypothetical protein	-
CSC38_RS21565	4008833..4009051	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
CSC38_RS21570	4009236..4009685	-	450	WP_032140890.1	hypothetical protein	-
CSC38_RS21575	4010001..4010816	-	816	WP_112032776.1	hypothetical protein	-
CSC38_RS21580	4010860..4011557	-	698	Protein_3834	IS1 family transposase	-
CSC38_RS21590	4011915..4012157	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T107765 WP_000897302.1 NZ_CP030337:4007197-4007508 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T107765 NZ_CP030337:4007197-4007508 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT107765 WP_000356397.1 NZ_CP030337:4007509-4007799 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT107765 NZ_CP030337:4007509-4007799 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416