Detailed information of TA system TA107759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1893604..1894106
• Replicon: chromosome
• Accession: NZ_CP030337
• Organism: Escherichia coli strain AR_451

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: CSC38_RS10975
• Protein ID: WP_000767819.1
• Coordinates: 1893604..1893858 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: CSC38_RS10980
• Protein ID: WP_001259253.1
• Coordinates: 1893855..1894106 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC38_RS10945	1889422..1889880	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
CSC38_RS10955	1890097..1891455	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
CSC38_RS10960	1891445..1891507	-	63	WP_010723108.1	membrane protein YoeI	-
CSC38_RS10965	1891722..1892651	-	930	WP_000803366.1	LysR family transcriptional regulator	-
CSC38_RS10970	1892697..1893521	-	825	WP_000754737.1	SDR family oxidoreductase	-
CSC38_RS10975	1893604..1893858	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
CSC38_RS10980	1893855..1894106	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
CSC38_RS10990	1894389..1894439	+	51	WP_001364200.1	his operon leader peptide	-
CSC38_RS11000	1894585..1895484	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
CSC38_RS11005	1895490..1896794	+	1305	WP_001296211.1	histidinol dehydrogenase	-
CSC38_RS11010	1896791..1897861	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
CSC38_RS11015	1897861..1898928	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1889422..1889880	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T107759 WP_000767819.1 NZ_CP030337:c1893858-1893604 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T107759 NZ_CP030337:c1893858-1893604 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT107759 WP_001259253.1 NZ_CP030337:c1894106-1893855 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT107759 NZ_CP030337:c1894106-1893855 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3