Detailed information of TA system TA107744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 225599..226278
• Replicon: chromosome
• Accession: NZ_CP030337
• Organism: Escherichia coli strain AR_451

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: CSC38_RS01855
• Protein ID: WP_000057523.1
• Coordinates: 225599..225901 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: CSC38_RS01860
• Protein ID: WP_000806442.1
• Coordinates: 225937..226278 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC38_RS01825	220975..222627	+	1653	WP_000771748.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
CSC38_RS01830	222665..223168	-	504	WP_000667000.1	hypothetical protein	-
CSC38_RS01835	223165..223965	-	801	WP_000439798.1	hypothetical protein	-
CSC38_RS01840	223989..224468	-	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
CSC38_RS01850	224672..225466	-	795	WP_000365147.1	TraB/GumN family protein	-
CSC38_RS01855	225599..225901	+	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC38_RS01860	225937..226278	+	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
CSC38_RS01865	226336..228840	-	2505	WP_000083947.1	copper-exporting P-type ATPase CopA	-
CSC38_RS01870	229102..230034	+	933	WP_000883041.1	glutaminase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T107744 WP_000057523.1 NZ_CP030337:225599-225901 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T107744 NZ_CP030337:225599-225901 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT107744 WP_000806442.1 NZ_CP030337:225937-226278 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT107744 NZ_CP030337:225937-226278 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3