Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1777545..1778047 Replicon chromosome
Accession NZ_CP030331
Organism Escherichia coli strain AR_452

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CSC39_RS09955 Protein ID WP_000767829.1
Coordinates 1777793..1778047 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CSC39_RS09950 Protein ID WP_001259255.1
Coordinates 1777545..1777796 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC39_RS09920 1772723..1773790 - 1068 WP_000080063.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CSC39_RS09925 1773790..1774860 - 1071 WP_000108962.1 histidinol-phosphate transaminase -
CSC39_RS09930 1774857..1776161 - 1305 WP_045172205.1 histidinol dehydrogenase -
CSC39_RS09935 1776167..1777066 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CSC39_RS09945 1777212..1777262 - 51 WP_001364200.1 his operon leader peptide -
CSC39_RS09950 1777545..1777796 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CSC39_RS09955 1777793..1778047 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CSC39_RS09960 1778130..1778954 + 825 WP_000754749.1 SDR family oxidoreductase -
CSC39_RS09965 1779000..1779929 + 930 WP_000803360.1 LysR family transcriptional regulator -
CSC39_RS09970 1780144..1780206 + 63 WP_010723108.1 membrane protein YoeI -
CSC39_RS09975 1780196..1781554 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CSC39_RS09980 1781733..1782791 + 1059 WP_000492340.1 transport protein YeeE -
CSC39_RS09985 1782805..1783032 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T107725 WP_000767829.1 NZ_CP030331:1777793-1778047 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T107725 NZ_CP030331:1777793-1778047 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT107725 WP_001259255.1 NZ_CP030331:1777545-1777796 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT107725 NZ_CP030331:1777545-1777796 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References