Detailed information of TA system TA107721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 586848..587647
• Replicon: chromosome
• Accession: NZ_CP030331
• Organism: Escherichia coli strain AR_452

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: CSC39_RS03995
• Protein ID: WP_000347266.1
• Coordinates: 586848..587312 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: CSC39_RS04000
• Protein ID: WP_001307405.1
• Coordinates: 587312..587647 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC39_RS03960	581849..582283	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
CSC39_RS03965	582301..583179	-	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CSC39_RS03970	583169..583948	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CSC39_RS03975	583959..584432	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CSC39_RS03980	584455..585735	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CSC39_RS03990	585984..586793	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CSC39_RS03995	586848..587312	-	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CSC39_RS04000	587312..587647	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CSC39_RS04005	587796..589367	-	1572	WP_001273741.1	galactarate dehydratase	-
CSC39_RS04010	589742..591076	+	1335	WP_000599640.1	galactarate/glucarate/glycerate transporter GarP	-
CSC39_RS04015	591092..591862	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	586848..598303	11455

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T107721 WP_000347266.1 NZ_CP030331:c587312-586848 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T107721 NZ_CP030331:c587312-586848 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT107721 WP_001307405.1 NZ_CP030331:c587647-587312 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT107721 NZ_CP030331:c587647-587312 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7