Detailed information of TA system TA107716

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 121766..122020
• Replicon: plasmid unnamed1
• Accession: NZ_CP030329
• Organism: Escherichia coli strain AR_452

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CSC39_RS00805
• Protein ID: WP_032215358.1
• Coordinates: 121814..122020 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	121766..121827 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC39_RS00770	117526..118272	+	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-
CSC39_RS00775	118327..118887	+	561	WP_001567328.1	fertility inhibition protein FinO	-
CSC39_RS00780	119018..119227	+	210	WP_039023209.1	hypothetical protein	-
CSC39_RS00785	119639..120265	+	627	WP_029702149.1	hypothetical protein	-
CSC39_RS00790	120417..120986	+	570	WP_112021708.1	DUF2726 domain-containing protein	-
CSC39_RS26000	121361..121627	+	267	WP_087757657.1	hypothetical protein	-
-	121766..121827	-	62	NuclAT_0	-	Antitoxin
-	121766..121827	-	62	NuclAT_0	-	Antitoxin
-	121766..121827	-	62	NuclAT_0	-	Antitoxin
-	121766..121827	-	62	NuclAT_0	-	Antitoxin
CSC39_RS00805	121814..122020	+	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
CSC39_RS00810	122304..122561	+	258	WP_000083833.1	replication regulatory protein RepA	-
CSC39_RS00820	122797..122871	+	75	WP_001365705.1	RepA leader peptide Tap	-
CSC39_RS00825	122864..123721	+	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
CSC39_RS00835	124660..125313	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
CSC39_RS25710	125406..125663	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
CSC39_RS00840	125596..125997	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
CSC39_RS00855	126246..126661	+	416	Protein_143	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qepA4 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / mph(A) / blaTEM-1B / tet(B)	-	1..128762	128762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T107716 WP_032215358.1 NZ_CP030329:121814-122020 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T107716 NZ_CP030329:121814-122020 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT107716 NZ_CP030329:c121827-121766 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin