Detailed information of TA system TA107709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4088459..4089054
• Replicon: chromosome
• Accession: NZ_CP030327
• Organism: Pseudomonas aeruginosa strain AR_458

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CSC44_RS19465
• Protein ID: WP_003113526.1
• Coordinates: 4088459..4088737 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CSC44_RS19470
• Protein ID: WP_003099268.1
• Coordinates: 4088749..4089054 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC44_RS19445	4083885..4085123	+	1239	WP_014603324.1	C69 family dipeptidase	-
CSC44_RS19450	4085185..4085832	+	648	WP_003095021.1	carbonate dehydratase	-
CSC44_RS19455	4085902..4088130	-	2229	WP_031633241.1	TonB-dependent receptor	-
CSC44_RS19465	4088459..4088737	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC44_RS19470	4088749..4089054	+	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
CSC44_RS19480	4089460..4090560	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
CSC44_RS19485	4090601..4091185	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CSC44_RS19490	4091227..4091841	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
CSC44_RS19495	4091958..4092899	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CSC44_RS19505	4093066..4093914	-	849	WP_023115601.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T107709 WP_003113526.1 NZ_CP030327:4088459-4088737 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T107709 NZ_CP030327:4088459-4088737 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT107709 WP_003099268.1 NZ_CP030327:4088749-4089054 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT107709 NZ_CP030327:4088749-4089054 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3