Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3808176..3808773 Replicon chromosome
Accession NZ_CP030302
Organism Klebsiella pneumoniae strain 205880

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag DRB04_RS18610 Protein ID WP_004142563.1
Coordinates 3808456..3808773 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag DRB04_RS18605 Protein ID WP_004142561.1
Coordinates 3808176..3808463 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB04_RS18575 (DRB04_18975) 3804257..3804505 + 249 WP_002893037.1 DUF1158 domain-containing protein -
DRB04_RS18580 (DRB04_18980) 3804523..3804864 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
DRB04_RS18585 (DRB04_18985) 3804895..3806010 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
DRB04_RS18590 (DRB04_18990) 3806189..3806770 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
DRB04_RS18595 (DRB04_18995) 3806770..3807138 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
DRB04_RS18600 (DRB04_19000) 3807258..3807911 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
DRB04_RS18605 (DRB04_19005) 3808176..3808463 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
DRB04_RS18610 (DRB04_19010) 3808456..3808773 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DRB04_RS18615 (DRB04_19015) 3808958..3810001 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
DRB04_RS18620 (DRB04_19025) 3810668..3811534 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
DRB04_RS18625 (DRB04_19030) 3811643..3813070 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T107574 WP_004142563.1 NZ_CP030302:c3808773-3808456 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T107574 NZ_CP030302:c3808773-3808456 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT107574 WP_004142561.1 NZ_CP030302:c3808463-3808176 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT107574 NZ_CP030302:c3808463-3808176 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References