Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 113001..113254 Replicon plasmid p283747-1
Accession NZ_CP030301
Organism Klebsiella pneumoniae strain 283747

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag DRB02_RS27860 Protein ID WP_001312851.1
Coordinates 113001..113150 (-) Length 50 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 113195..113254 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB02_RS27825 (108360) 108360..108775 - 416 Protein_150 IS1-like element IS1B family transposase -
DRB02_RS27830 (109024) 109024..109425 - 402 WP_001398199.1 type II toxin-antitoxin system toxin endoribonuclease PemK -
DRB02_RS27835 (109358) 109358..109615 - 258 WP_000557619.1 type II toxin-antitoxin system antitoxin PemI -
DRB02_RS27840 (109708) 109708..110361 - 654 WP_000616807.1 CPBP family intramembrane metalloprotease -
DRB02_RS27845 (111300) 111300..112157 - 858 WP_032495102.1 incFII family plasmid replication initiator RepA -
DRB02_RS28340 (112176) 112176..112354 - 179 Protein_155 protein CopA/IncA -
DRB02_RS27855 (112469) 112469..112717 - 249 WP_000083837.1 replication regulatory protein RepA -
DRB02_RS27860 (113001) 113001..113150 - 150 WP_001312851.1 Hok/Gef family protein Toxin
- (113195) 113195..113254 + 60 NuclAT_1 - Antitoxin
- (113195) 113195..113254 + 60 NuclAT_1 - Antitoxin
- (113195) 113195..113254 + 60 NuclAT_1 - Antitoxin
- (113195) 113195..113254 + 60 NuclAT_1 - Antitoxin
DRB02_RS27865 (113455) 113455..113787 - 333 WP_152916585.1 hypothetical protein -
DRB02_RS27870 (113849) 113849..114448 - 600 WP_032083981.1 PIN domain-containing protein -
DRB02_RS27875 (114834) 114834..115034 - 201 WP_015059022.1 hypothetical protein -
DRB02_RS27880 (115166) 115166..115726 - 561 WP_000139328.1 fertility inhibition protein FinO -
DRB02_RS27885 (115781) 115781..116527 - 747 WP_000205770.1 conjugal transfer pilus acetylase TraX -
DRB02_RS27890 (116547) 116547..116747 - 201 WP_072354025.1 hypothetical protein -
DRB02_RS27895 (116772) 116772..117476 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
DRB02_RS27900 (117529) 117529..117594 + 66 Protein_165 helix-turn-helix domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 - 1..148021 148021


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-45)


Sequences


Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T107557 WP_001312851.1 NZ_CP030301:c113150-113001 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T107557 NZ_CP030301:c113150-113001 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 60 bp

>AT107557 NZ_CP030301:113195-113254 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829C8K5


Antitoxin

Download structure file

References