Detailed information of TA system TA107554

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74927..75353
• Replicon: plasmid p283747-1
• Accession: NZ_CP030301
• Organism: Klebsiella pneumoniae strain 283747

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: DRB02_RS27615
• Protein ID: WP_001372321.1
• Coordinates: 74927..75052 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	75129..75353 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRB02_RS27580 (69982)	69982..70209	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
DRB02_RS27585 (70303)	70303..70989	-	687	WP_015059009.1	PAS domain-containing protein	-
DRB02_RS27590 (71180)	71180..71563	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
DRB02_RS27595 (71840)	71840..72487	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
DRB02_RS27600 (72784)	72784..73605	-	822	WP_001234445.1	DUF932 domain-containing protein	-
DRB02_RS27605 (73716)	73716..74012	-	297	WP_001272251.1	hypothetical protein	-
DRB02_RS27610 (74312)	74312..74608	+	297	Protein_105	hypothetical protein	-
DRB02_RS27615 (74927)	74927..75052	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
DRB02_RS28320 (74994)	74994..75143	-	150	Protein_107	plasmid maintenance protein Mok	-
- (75129)	75129..75353	-	225	NuclAT_0	-	Antitoxin
- (75129)	75129..75353	-	225	NuclAT_0	-	Antitoxin
- (75129)	75129..75353	-	225	NuclAT_0	-	Antitoxin
- (75129)	75129..75353	-	225	NuclAT_0	-	Antitoxin
DRB02_RS27620 (75365)	75365..76084	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
DRB02_RS27625 (76081)	76081..76515	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
DRB02_RS27630 (76584)	76584..78607	-	2024	Protein_110	ParB/RepB/Spo0J family partition protein	-
DRB02_RS27635 (78668)	78668..78901	-	234	WP_000006003.1	DUF905 family protein	-
DRB02_RS27640 (78959)	78959..79486	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
DRB02_RS28325 (79788)	79788..80243	+	456	Protein_113	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65	-	1..148021	148021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T107554 WP_001372321.1 NZ_CP030301:c75052-74927 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T107554 NZ_CP030301:c75052-74927 [Klebsiella pneumoniae]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT107554 NZ_CP030301:c75353-75129 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin