Detailed information of TA system TA107531

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27998..28267
• Replicon: plasmid p309074-2
• Accession: NZ_CP030298
• Organism: Klebsiella pneumoniae strain 309074

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: DQG43_RS26955
• Protein ID: WP_001372321.1
• Coordinates: 28142..28267 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27998..28063 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQG43_RS27760	23004..23465	-	462	Protein_33	hypothetical protein	-
DQG43_RS26930	23767..24294	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
DQG43_RS26935	24352..24585	+	234	WP_000006003.1	DUF905 family protein	-
DQG43_RS26940	24646..26610	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
DQG43_RS26945	26679..27113	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
DQG43_RS26950	27110..27829	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	27841..28065	+	225	NuclAT_0	-	-
-	27841..28065	+	225	NuclAT_0	-	-
-	27841..28065	+	225	NuclAT_0	-	-
-	27841..28065	+	225	NuclAT_0	-	-
-	27998..28063	+	66	-	-	Antitoxin
DQG43_RS27765	28051..28200	+	150	Protein_39	plasmid maintenance protein Mok	-
DQG43_RS26955	28142..28267	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
DQG43_RS26960	28568..28864	-	297	Protein_41	hypothetical protein	-
DQG43_RS26965	29164..29460	+	297	WP_001272251.1	hypothetical protein	-
DQG43_RS26970	29571..30392	+	822	WP_001234445.1	DUF932 domain-containing protein	-
DQG43_RS26975	30689..31279	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
DQG43_RS26980	31612..31995	+	384	WP_001151529.1	conjugal transfer relaxosome DNA-binding protein TraM	-
DQG43_RS26985	32187..32834	+	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
DQG43_RS26990	32954..33181	+	228	WP_000589556.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..70438	70438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T107531 WP_001372321.1 NZ_CP030298:28142-28267 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T107531 NZ_CP030298:28142-28267 [Klebsiella pneumoniae]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT107531 NZ_CP030298:27998-28063 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin