Detailed information of TA system TA107478

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 88001..88254
• Replicon: plasmid pLKSZ02
• Accession: NZ_CP030283
• Organism: Escherichia coli strain E308

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: DQW89_RS26430
• Protein ID: WP_001312851.1
• Coordinates: 88001..88150 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	88195..88254 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW89_RS26380	83361..83776	-	416	Protein_110	IS1 family transposase	-
DQW89_RS26395	84025..84426	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
DQW89_RS27970	84359..84616	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
DQW89_RS26400	84709..85362	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
DQW89_RS27975	85460..85600	-	141	WP_001333237.1	hypothetical protein	-
DQW89_RS26410	86285..87157	-	873	WP_162883675.1	incFII family plasmid replication initiator RepA	-
DQW89_RS26415	87150..87224	-	75	WP_001375168.1	RepA leader peptide Tap	-
DQW89_RS26425	87469..87717	-	249	WP_000083837.1	replication regulatory protein RepA	-
DQW89_RS26430	88001..88150	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	88195..88254	+	60	NuclAT_1	-	Antitoxin
-	88195..88254	+	60	NuclAT_1	-	Antitoxin
-	88195..88254	+	60	NuclAT_1	-	Antitoxin
-	88195..88254	+	60	NuclAT_1	-	Antitoxin
DQW89_RS26440	88455..88685	-	231	WP_001736714.1	hypothetical protein	-
DQW89_RS26445	88849..89448	-	600	WP_032083981.1	hypothetical protein	-
DQW89_RS26450	89834..90034	-	201	WP_015059022.1	hypothetical protein	-
DQW89_RS26455	90166..90726	-	561	WP_000139328.1	fertility inhibition protein FinO	-
DQW89_RS26460	90781..91527	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / fosA3 / oqxA / oqxB / aph(3')-IIa / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..113645	113645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T107478 WP_001312851.1 NZ_CP030283:c88150-88001 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T107478 NZ_CP030283:c88150-88001 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT107478 NZ_CP030283:88195-88254 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin