Detailed information of TA system TA107473

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13787..14213
• Replicon: plasmid pLKSZ02
• Accession: NZ_CP030283
• Organism: Escherichia coli strain E308

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: DQW89_RS25875
• Protein ID: WP_001312861.1
• Coordinates: 13787..13945 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	13989..14213 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW89_RS25830	8846..9073	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
DQW89_RS25835	9167..9853	-	687	WP_015059009.1	PAS domain-containing protein	-
DQW89_RS25840	10044..10426	-	383	Protein_20	relaxosome protein TraM	-
DQW89_RS25845	10703..11350	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
DQW89_RS25850	11646..12467	-	822	WP_001234445.1	DUF945 domain-containing protein	-
DQW89_RS25855	12576..12872	-	297	WP_001272251.1	hypothetical protein	-
DQW89_RS28125	13172..13549	+	378	Protein_24	hypothetical protein	-
DQW89_RS25875	13787..13945	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	13989..14213	-	225	NuclAT_0	-	Antitoxin
-	13989..14213	-	225	NuclAT_0	-	Antitoxin
-	13989..14213	-	225	NuclAT_0	-	Antitoxin
-	13989..14213	-	225	NuclAT_0	-	Antitoxin
DQW89_RS27950	14025..14204	+	180	WP_001309233.1	hypothetical protein	-
DQW89_RS25880	14225..14944	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
DQW89_RS25885	14941..15375	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
DQW89_RS25890	15444..17467	-	2024	Protein_29	ParB/RepB/Spo0J family partition protein	-
DQW89_RS25895	17528..17761	-	234	WP_000006003.1	DUF905 family protein	-
DQW89_RS25900	17819..18346	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / fosA3 / oqxA / oqxB / aph(3')-IIa / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..113645	113645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T107473 WP_001312861.1 NZ_CP030283:c13945-13787 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T107473 NZ_CP030283:c13945-13787 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT107473 NZ_CP030283:c14213-13989 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin