Detailed information of TA system TA107468

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40871..41135
• Replicon: plasmid pLKSZ01
• Accession: NZ_CP030282
• Organism: Escherichia coli strain E308

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: DQW89_RS25255
• Protein ID: WP_001387489.1
• Coordinates: 40871..41023 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	41073..41135 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW89_RS25235	36148..38316	+	2169	WP_000698357.1	DotA/TraY family protein	-
DQW89_RS25240	38390..39040	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
DQW89_RS25245	39112..39321	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
DQW89_RS27900	39713..39889	+	177	WP_001054898.1	hypothetical protein	-
DQW89_RS25250	40548..40799	+	252	WP_001291964.1	hypothetical protein	-
DQW89_RS25255	40871..41023	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	41073..41135	+	63	NuclAT_0	-	Antitoxin
-	41073..41135	+	63	NuclAT_0	-	Antitoxin
-	41073..41135	+	63	NuclAT_0	-	Antitoxin
-	41073..41135	+	63	NuclAT_0	-	Antitoxin
DQW89_RS25260	41315..42523	+	1209	WP_039005623.1	IncI1-type conjugal transfer protein TrbA	-
DQW89_RS25265	42542..43612	+	1071	WP_039005625.1	IncI1-type conjugal transfer protein TrbB	-
DQW89_RS25270	43605..45896	+	2292	WP_072742854.1	conjugal transfer protein TrbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-VIII	1..111721	111721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T107468 WP_001387489.1 NZ_CP030282:c41023-40871 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T107468 NZ_CP030282:c41023-40871 [Escherichia coli]
ATGCCACAGCGAACATTTTTAATGATGTTGATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGGCTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT107468 NZ_CP030282:41073-41135 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin