Detailed information of TA system TA107465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3740167..3740861
• Replicon: chromosome
• Accession: NZ_CP030281
• Organism: Escherichia coli strain E308

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: DQW89_RS19695
• Protein ID: WP_001263493.1
• Coordinates: 3740167..3740565 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: DQW89_RS19700
• Protein ID: WP_000554757.1
• Coordinates: 3740568..3740861 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW89_RS19665	3735167..3736411	-	1245	WP_000189541.1	esterase FrsA	-
-	3735827..3735907	-	81	NuclAT_12	-	-
-	3735827..3735907	-	81	NuclAT_12	-	-
-	3735827..3735907	-	81	NuclAT_12	-	-
-	3735827..3735907	-	81	NuclAT_12	-	-
DQW89_RS19670	3736503..3736961	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DQW89_RS19675	3737222..3738679	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
DQW89_RS19680	3738736..3739257	-	522	Protein_3597	peptide chain release factor H	-
DQW89_RS19685	3739256..3739459	-	204	Protein_3598	RNA ligase RtcB family protein	-
DQW89_RS19690	3739705..3740157	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
DQW89_RS19695	3740167..3740565	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DQW89_RS19700	3740568..3740861	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DQW89_RS19705	3740913..3741967	-	1055	Protein_3602	DNA polymerase IV	-
DQW89_RS19710	3742038..3742823	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
DQW89_RS19715	3742795..3744507	+	1713	Protein_3604	flagellar biosynthesis protein FlhA	-
DQW89_RS19720	3744731..3745228	-	498	WP_072778518.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T107465 WP_001263493.1 NZ_CP030281:c3740565-3740167 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T107465 NZ_CP030281:c3740565-3740167 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT107465 WP_000554757.1 NZ_CP030281:c3740861-3740568 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT107465 NZ_CP030281:c3740861-3740568 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6