Detailed information of TA system TA107442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 56785..57512
• Replicon: plasmid pSC7-vir
• Accession: NZ_CP030270
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain SC-7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: DQQ08_RS27570
• Protein ID: WP_011251285.1
• Coordinates: 57201..57512 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DQQ08_RS27565
• Protein ID: WP_011251286.1
• Coordinates: 56785..57204 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQQ08_RS27555	54671..55390	+	720	Protein_48	hypothetical protein	-
DQQ08_RS27560	55765..56638	-	874	Protein_49	transposase	-
DQQ08_RS27565	56785..57204	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
DQQ08_RS27570	57201..57512	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
DQQ08_RS27575	57717..58175	-	459	Protein_52	DDE-type integrase/transposase/recombinase	-
DQQ08_RS27580	58289..58986	+	698	Protein_53	IS1 family transposase	-
DQQ08_RS27585	59003..59437	+	435	Protein_54	thioredoxin fold domain-containing protein	-
DQQ08_RS27590	59454..59765	+	312	WP_011251282.1	hypothetical protein	-
DQQ08_RS27595	59779..60120	+	342	WP_011251281.1	hypothetical protein	-
DQQ08_RS27600	60180..61136	+	957	WP_011251280.1	DsbA family protein	-
DQQ08_RS27605	61253..61828	+	576	WP_074424927.1	hypothetical protein	-
DQQ08_RS27610	61936..62451	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sul1 / qacE / ant(3'')-Ia / cmlA1 / aadA2	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / pla	1..236809	236809
	-	inside	IScluster/Tn	-	-	56243..58986	2743

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T107442 WP_011251285.1 NZ_CP030270:c57512-57201 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T107442 NZ_CP030270:c57512-57201 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT107442 WP_011251286.1 NZ_CP030270:c57204-56785 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT107442 NZ_CP030270:c57204-56785 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1