Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4219479..4219981 Replicon chromosome
Accession NZ_CP030240
Organism Escherichia coli strain ER1709

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DQ225_RS20675 Protein ID WP_000767829.1
Coordinates 4219479..4219733 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DQ225_RS20680 Protein ID WP_001259255.1
Coordinates 4219730..4219981 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQ225_RS20645 4214495..4214722 - 228 WP_000234896.1 sulfurtransferase TusA -
DQ225_RS20650 4214736..4215794 - 1059 WP_000492339.1 transport protein YeeE -
DQ225_RS20655 4215972..4217330 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DQ225_RS20660 4217320..4217382 - 63 WP_010723108.1 membrane protein YoeI -
DQ225_RS20665 4217597..4218526 - 930 WP_000803351.1 LysR family transcriptional regulator -
DQ225_RS20670 4218572..4219396 - 825 WP_000754737.1 SDR family oxidoreductase -
DQ225_RS20675 4219479..4219733 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DQ225_RS20680 4219730..4219981 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DQ225_RS20685 4220264..4220314 + 51 WP_001364200.1 his operon leader peptide -
DQ225_RS20690 4220460..4221359 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DQ225_RS20695 4221365..4222669 + 1305 WP_000009594.1 histidinol dehydrogenase -
DQ225_RS20700 4222666..4223736 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
DQ225_RS20705 4223736..4224803 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T107401 WP_000767829.1 NZ_CP030240:c4219733-4219479 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T107401 NZ_CP030240:c4219733-4219479 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT107401 WP_001259255.1 NZ_CP030240:c4219981-4219730 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT107401 NZ_CP030240:c4219981-4219730 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References