107401

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4219479..4219981 Replicon chromosome
Accession NZ_CP030240
Organism Escherichia coli strain ER1709

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DQ225_RS20675 Protein ID WP_000767829.1
Coordinates 4219479..4219733 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DQ225_RS20680 Protein ID WP_001259255.1
Coordinates 4219730..4219981 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQ225_RS20645 4214495..4214722 - 228 WP_000234896.1 sulfurtransferase TusA -
DQ225_RS20650 4214736..4215794 - 1059 WP_000492339.1 transport protein YeeE -
DQ225_RS20655 4215972..4217330 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DQ225_RS20660 4217320..4217382 - 63 WP_010723108.1 membrane protein YoeI -
DQ225_RS20665 4217597..4218526 - 930 WP_000803351.1 LysR family transcriptional regulator -
DQ225_RS20670 4218572..4219396 - 825 WP_000754737.1 SDR family oxidoreductase -
DQ225_RS20675 4219479..4219733 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DQ225_RS20680 4219730..4219981 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DQ225_RS20685 4220264..4220314 + 51 WP_001364200.1 his operon leader peptide -
DQ225_RS20690 4220460..4221359 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DQ225_RS20695 4221365..4222669 + 1305 WP_000009594.1 histidinol dehydrogenase -
DQ225_RS20700 4222666..4223736 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
DQ225_RS20705 4223736..4224803 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T107401 WP_000767829.1 NZ_CP030240:c4219733-4219479 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T107401 NZ_CP030240:c4219733-4219479 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT107401 WP_001259255.1 NZ_CP030240:c4219981-4219730 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT107401 NZ_CP030240:c4219981-4219730 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References