Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2430761..2431455 Replicon chromosome
Accession NZ_CP030240
Organism Escherichia coli strain ER1709

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag DQ225_RS11650 Protein ID WP_001263489.1
Coordinates 2431057..2431455 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag DQ225_RS11645 Protein ID WP_000554758.1
Coordinates 2430761..2431054 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQ225_RS11625 2426393..2426890 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
DQ225_RS11630 2427114..2428826 - 1713 Protein_2246 flagellar biosynthesis protein FlhA -
DQ225_RS11635 2428798..2429583 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
DQ225_RS11640 2429654..2430709 + 1056 WP_001226164.1 DNA polymerase IV -
DQ225_RS11645 2430761..2431054 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
DQ225_RS11650 2431057..2431455 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
DQ225_RS11655 2431465..2431917 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
DQ225_RS11660 2432235..2432441 + 207 Protein_2252 RtcB family protein -
DQ225_RS11665 2432437..2432958 + 522 Protein_2253 peptide chain release factor H -
DQ225_RS11670 2433015..2434472 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
DQ225_RS11675 2434733..2435191 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 2435787..2435867 + 81 NuclAT_12 - -
- 2435787..2435867 + 81 NuclAT_12 - -
- 2435787..2435867 + 81 NuclAT_12 - -
- 2435787..2435867 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T107376 WP_001263489.1 NZ_CP030240:2431057-2431455 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T107376 NZ_CP030240:2431057-2431455 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT107376 WP_000554758.1 NZ_CP030240:2430761-2431054 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT107376 NZ_CP030240:2430761-2431054 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References