Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 5009981..5010567 Replicon chromosome
Accession NZ_CP030174
Organism Klebsiella pneumoniae subsp. pneumoniae strain 11219

Toxin (Protein)


Gene name doc Uniprot ID -
Locus tag DQB71_RS25525 Protein ID WP_021312828.1
Coordinates 5009981..5010349 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag DQB71_RS25530 Protein ID WP_004174006.1
Coordinates 5010346..5010567 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQB71_RS25505 5005484..5006554 - 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
DQB71_RS25510 5006556..5007401 - 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
DQB71_RS25515 5007398..5008285 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
DQB71_RS25520 5008392..5009708 - 1317 WP_021312827.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
DQB71_RS25525 5009981..5010349 - 369 WP_021312828.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DQB71_RS25530 5010346..5010567 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DQB71_RS25535 5010731..5011444 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
DQB71_RS25540 5011446..5012213 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
DQB71_RS25545 5012210..5013487 - 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
DQB71_RS25550 5013484..5014410 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 5004747..5013487 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13578.96 Da        Isoelectric Point: 8.5493

>T107330 WP_021312828.1 NZ_CP030174:c5010349-5009981 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGYIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T107330 NZ_CP030174:c5010349-5009981 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTTATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT107330 WP_004174006.1 NZ_CP030174:c5010567-5010346 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT107330 NZ_CP030174:c5010567-5010346 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References