Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4983732..4984378 Replicon chromosome
Accession NZ_CP030174
Organism Klebsiella pneumoniae subsp. pneumoniae strain 11219

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag DQB71_RS25415 Protein ID WP_004174016.1
Coordinates 4984031..4984378 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4S7L580
Locus tag DQB71_RS25410 Protein ID WP_019725405.1
Coordinates 4983732..4984031 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQB71_RS25390 4979938..4980696 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
DQB71_RS25395 4980746..4981576 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
DQB71_RS25400 4981627..4981956 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
DQB71_RS25405 4982161..4983669 + 1509 WP_065889107.1 glycerol-3-phosphate dehydrogenase -
DQB71_RS25410 4983732..4984031 - 300 WP_019725405.1 XRE family transcriptional regulator Antitoxin
DQB71_RS25415 4984031..4984378 - 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DQB71_RS25420 4984554..4987001 - 2448 WP_002920561.1 glycogen phosphorylase -
DQB71_RS25425 4987019..4988452 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T107329 WP_004174016.1 NZ_CP030174:c4984378-4984031 [Klebsiella pneumoniae subsp. pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T107329 NZ_CP030174:c4984378-4984031 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10775.53 Da        Isoelectric Point: 9.7681

>AT107329 WP_019725405.1 NZ_CP030174:c4984031-4983732 [Klebsiella pneumoniae subsp. pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKSYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT107329 NZ_CP030174:c4984031-4983732 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A4S7L580

References