Detailed information of TA system TA107318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1404422..1405019
• Replicon: chromosome
• Accession: NZ_CP030174
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain 11219

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: DQB71_RS07015
• Protein ID: WP_004893639.1
• Coordinates: 1404422..1404739 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: DQB71_RS07020
• Protein ID: WP_004142561.1
• Coordinates: 1404732..1405019 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQB71_RS06995	1400126..1401553	-	1428	WP_065889794.1	MFS transporter	-
DQB71_RS07000	1401662..1402528	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
DQB71_RS07010	1403194..1404237	+	1044	WP_021314069.1	DUF2157 domain-containing protein	-
DQB71_RS07015	1404422..1404739	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DQB71_RS07020	1404732..1405019	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
DQB71_RS07025	1405284..1405937	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
DQB71_RS07030	1406057..1406425	-	369	WP_002893024.1	MmcQ/YjbR family DNA-binding protein	-
DQB71_RS07035	1406422..1407006	-	585	WP_002893026.1	TetR/AcrR family transcriptional regulator	-
DQB71_RS07040	1407186..1408301	+	1116	WP_020316605.1	MBL fold metallo-hydrolase	-
DQB71_RS07045	1408332..1408673	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
DQB71_RS07050	1408691..1408939	-	249	WP_065889795.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T107318 WP_004893639.1 NZ_CP030174:1404422-1404739 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T107318 NZ_CP030174:1404422-1404739 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT107318 WP_004142561.1 NZ_CP030174:1404732-1405019 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT107318 NZ_CP030174:1404732-1405019 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1