Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 19053..19317 Replicon plasmid pJSMCR1_3
Accession NZ_CP030155
Organism Escherichia coli strain JSMCR1

Toxin (Protein)


Gene name pndA Uniprot ID U9Z417
Locus tag JSMCR1_RS24895 Protein ID WP_001387489.1
Coordinates 19053..19205 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 19255..19317 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSMCR1_RS24865 (14330) 14330..16498 + 2169 WP_000698357.1 DotA/TraY family protein -
JSMCR1_RS24870 (16572) 16572..17222 + 651 WP_001178506.1 plasmid IncI1-type surface exclusion protein ExcA -
JSMCR1_RS24875 (17294) 17294..17503 - 210 WP_000062603.1 HEAT repeat domain-containing protein -
JSMCR1_RS24880 (17895) 17895..18071 + 177 WP_001054898.1 hypothetical protein -
JSMCR1_RS24885 (18136) 18136..18368 - 233 Protein_18 hypothetical protein -
JSMCR1_RS24890 (18730) 18730..18981 + 252 WP_001291964.1 hypothetical protein -
JSMCR1_RS24895 (19053) 19053..19205 - 153 WP_001387489.1 Hok/Gef family protein Toxin
- (19255) 19255..19317 + 63 NuclAT_0 - Antitoxin
- (19255) 19255..19317 + 63 NuclAT_0 - Antitoxin
- (19255) 19255..19317 + 63 NuclAT_0 - Antitoxin
- (19255) 19255..19317 + 63 NuclAT_0 - Antitoxin
JSMCR1_RS24900 (19497) 19497..20705 + 1209 WP_039005623.1 IncI1-type conjugal transfer protein TrbA -
JSMCR1_RS24905 (20724) 20724..21794 + 1071 WP_039005625.1 IncI1-type conjugal transfer protein TrbB -
JSMCR1_RS24910 (21787) 21787..24078 + 2292 WP_072742854.1 F-type conjugative transfer protein TrbC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - afaC-I 1..109689 109689


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5761.10 Da        Isoelectric Point: 8.7948

>T107247 WP_001387489.1 NZ_CP030155:c19205-19053 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T107247 NZ_CP030155:c19205-19053 [Escherichia coli]
ATGCCACAGCGAACATTTTTAATGATGTTGATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGGCTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 63 bp

>AT107247 NZ_CP030155:19255-19317 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Z417

Antitoxin

Download structure file

References