Detailed information of TA system TA107242

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82705..83131
• Replicon: plasmid pJSMCR1_1
• Accession: NZ_CP030153
• Organism: Escherichia coli strain JSMCR1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JSMCR1_RS23715
• Protein ID: WP_001372321.1
• Coordinates: 82705..82830 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	82907..83131 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSMCR1_RS23675 (77733)	77733..78359	-	627	WP_000434930.1	hypothetical protein	-
JSMCR1_RS23680 (78359)	78359..80038	-	1680	WP_256625366.1	AAA family ATPase	-
JSMCR1_RS23685 (80097)	80097..80801	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JSMCR1_RS23690 (80865)	80865..81374	-	510	Protein_95	DUF945 domain-containing protein	-
JSMCR1_RS23695 (81496)	81496..81783	-	288	WP_000107535.1	hypothetical protein	-
JSMCR1_RS23700 (81808)	81808..82014	-	207	WP_000547939.1	hypothetical protein	-
JSMCR1_RS23705 (82084)	82084..82257	+	174	Protein_98	hypothetical protein	-
JSMCR1_RS23710 (82255)	82255..82485	-	231	WP_001426396.1	hypothetical protein	-
JSMCR1_RS23715 (82705)	82705..82830	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JSMCR1_RS23720 (82772)	82772..82921	-	150	Protein_101	plasmid maintenance protein Mok	-
- (82907)	82907..83131	-	225	NuclAT_0	-	Antitoxin
- (82907)	82907..83131	-	225	NuclAT_0	-	Antitoxin
- (82907)	82907..83131	-	225	NuclAT_0	-	Antitoxin
- (82907)	82907..83131	-	225	NuclAT_0	-	Antitoxin
JSMCR1_RS23725 (82943)	82943..83131	+	189	WP_001299721.1	hypothetical protein	-
JSMCR1_RS23730 (83100)	83100..83862	-	763	Protein_103	plasmid SOS inhibition protein A	-
JSMCR1_RS23735 (83859)	83859..84293	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
JSMCR1_RS23740 (84348)	84348..86306	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
JSMCR1_RS23745 (86365)	86365..86598	-	234	WP_000006004.1	DUF905 domain-containing protein	-
JSMCR1_RS23750 (86654)	86654..87181	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
JSMCR1_RS23755 (87654)	87654..87914	-	261	WP_072132736.1	hypothetical protein	-
JSMCR1_RS23760 (87824)	87824..88066	-	243	WP_001610271.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / fosA3 / aac(3)-IId / blaCTX-M-65 / sitABCD	iroB / iroC / iroD / iroE / iroN	1..152677	152677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T107242 WP_001372321.1 NZ_CP030153:c82830-82705 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T107242 NZ_CP030153:c82830-82705 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT107242 NZ_CP030153:c83131-82907 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin