Detailed information of TA system TA107218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 11452..12053
• Replicon: plasmid p_Lmay_MDI272_tenre
• Accession: NZ_CP030149
• Organism: Leptospira mayottensis strain MDI272

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5F2EA92
• Locus tag: DQM68_RS19550
• Protein ID: WP_036047653.1
• Coordinates: 11452..11730 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5F2EA11
• Locus tag: DQM68_RS19555
• Protein ID: WP_036047650.1
• Coordinates: 11742..12053 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQM68_RS19505	7214..7933	+	720	WP_036047703.1	hypothetical protein	-
DQM68_RS19510	7940..8227	+	288	WP_002745862.1	hypothetical protein	-
DQM68_RS19515	8224..8919	+	696	WP_117344744.1	hypothetical protein	-
DQM68_RS19520	8940..9377	+	438	WP_002745880.1	hypothetical protein	-
DQM68_RS19525	9504..10271	-	768	Protein_11	ParB N-terminal domain-containing protein	-
DQM68_RS19530	10352..10486	+	135	WP_117344750.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DQM68_RS19535	10499..10792	+	294	WP_036047657.1	HigA family addiction module antidote protein	-
DQM68_RS19540	10907..11125	+	219	WP_002745829.1	hypothetical protein	-
DQM68_RS19545	11170..11382	+	213	WP_002745836.1	helix-turn-helix domain-containing protein	-
DQM68_RS19550	11452..11730	+	279	WP_036047653.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DQM68_RS19555	11742..12053	+	312	WP_036047650.1	HigA family addiction module antidote protein	Antitoxin
DQM68_RS19565	13414..13644	-	231	WP_162882195.1	hypothetical protein	-
DQM68_RS19570	13870..14573	-	704	Protein_19	helix-turn-helix domain-containing protein	-
DQM68_RS19575	14963..15515	-	553	Protein_20	DUF3102 domain-containing protein	-
DQM68_RS19580	15512..16233	-	722	Protein_21	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..52509	52509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-87)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10815.25 Da
• Isoelectric Point: 8.5988

>T107218 WP_036047653.1 NZ_CP030149:11452-11730 [Leptospira mayottensis]
MIISFKHKGLEQFFETGNRKGIQPDHAGKLGRILDRLDASLNPKDMNLPSFKLHQLKGTRQNRWSVWVNGNWRITFEFEG
ENAILVDYEDYH

Nucleotide

• Length: 279 bp

>T107218 NZ_CP030149:11452-11730 [Leptospira mayottensis]
TTGATAATTTCATTCAAACATAAAGGCCTGGAGCAATTTTTTGAGACCGGCAACAGGAAGGGAATTCAACCCGATCACGC
AGGTAAACTAGGTAGGATTTTGGATAGGTTGGACGCTTCATTAAATCCAAAGGATATGAACTTGCCCTCATTTAAATTGC
ATCAACTTAAGGGCACCAGACAAAATCGGTGGTCTGTTTGGGTAAATGGAAATTGGCGTATCACATTCGAATTTGAAGGT
GAAAATGCAATATTAGTCGATTACGAAGATTATCATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11278.16 Da
• Isoelectric Point: 10.4253

>AT107218 WP_036047650.1 NZ_CP030149:11742-12053 [Leptospira mayottensis]
MNKRKPTHPGEVLLEDVIKPLGLSITEAARDLGISRKTLSEIVNAKSSITPETAIRIALATNTSPEGWLNMQTKLDLWNA
LQDKPKNVIKFPISALSVLKSGF

Nucleotide

• Length: 312 bp

>AT107218 NZ_CP030149:11742-12053 [Leptospira mayottensis]
ATGAACAAAAGAAAACCAACACATCCAGGCGAAGTTTTATTAGAGGACGTAATCAAACCATTAGGTTTGTCCATAACAGA
AGCCGCGAGAGATCTGGGAATATCACGTAAAACGCTCTCGGAGATAGTTAATGCCAAGAGTTCGATCACTCCTGAAACGG
CTATTAGAATTGCATTAGCGACTAACACCTCTCCTGAGGGTTGGCTTAATATGCAAACTAAATTAGATTTATGGAATGCC
TTACAAGATAAACCTAAGAACGTGATTAAATTTCCGATATCTGCCCTGTCCGTTCTGAAATCTGGTTTTTAG

Structures

Toxin

• AlphaFold DB: A0A5F2EA92

Antitoxin

• AlphaFold DB: A0A5F2EA11