Detailed information of TA system TA107159

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29907..30160
• Replicon: plasmid pIncFII_L111
• Accession: NZ_CP030133
• Organism: Klebsiella pneumoniae strain 160111

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: DL890_RS29155
• Protein ID: WP_001312851.1
• Coordinates: 29907..30056 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	30101..30160 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DL890_RS29105	25266..25681	-	416	Protein_35	IS1 family transposase	-
DL890_RS29120	25930..26331	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
DL890_RS31420	26264..26521	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
DL890_RS29125	26614..27267	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
DL890_RS29135	28206..29063	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
DL890_RS29140	29056..29130	-	75	WP_001375168.1	RepA leader peptide Tap	-
DL890_RS29150	29375..29623	-	249	WP_000083837.1	replication regulatory protein RepA	-
DL890_RS29155	29907..30056	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	30101..30160	+	60	NuclAT_0	-	Antitoxin
-	30101..30160	+	60	NuclAT_0	-	Antitoxin
-	30101..30160	+	60	NuclAT_0	-	Antitoxin
-	30101..30160	+	60	NuclAT_0	-	Antitoxin
DL890_RS29165	30361..30591	-	231	WP_001736714.1	hypothetical protein	-
DL890_RS29170	30755..31354	-	600	WP_032083981.1	hypothetical protein	-
DL890_RS29175	31740..31940	-	201	WP_015059022.1	hypothetical protein	-
DL890_RS29180	32072..32632	-	561	WP_000139328.1	fertility inhibition protein FinO	-
DL890_RS29185	32687..33433	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
DL890_RS29190	33453..33614	-	162	Protein_48	DNA helicase	-
DL890_RS29195	33678..34381	+	704	Protein_49	IS6-like element IS26 family transposase	-
DL890_RS29200	34434..34499	+	66	Protein_50	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39248	39248

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T107159 WP_001312851.1 NZ_CP030133:c30056-29907 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T107159 NZ_CP030133:c30056-29907 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT107159 NZ_CP030133:30101-30160 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin