Detailed information of TA system TA107135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4716127..4716729
• Replicon: chromosome
• Accession: NZ_CP030111
• Organism: Escherichia coli strain MCJCHV-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: DPQ29_RS24370
• Protein ID: WP_000897302.1
• Coordinates: 4716418..4716729 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DPQ29_RS24365
• Protein ID: WP_000356397.1
• Coordinates: 4716127..4716417 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPQ29_RS24330	4712200..4713102	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
DPQ29_RS24335	4713099..4713734	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
DPQ29_RS24340	4713731..4714660	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
DPQ29_RS24345	4714876..4715094	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
DPQ29_RS24355	4715490..4715768	-	279	WP_001296612.1	hypothetical protein	-
DPQ29_RS24365	4716127..4716417	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
DPQ29_RS24370	4716418..4716729	-	312	WP_000897302.1	hypothetical protein	Toxin
DPQ29_RS24375	4716958..4717866	+	909	WP_001774092.1	alpha/beta hydrolase	-
DPQ29_RS24380	4717930..4718871	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DPQ29_RS24385	4718916..4719353	-	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DPQ29_RS24390	4719350..4720222	-	873	WP_000920762.1	virulence factor BrkB family protein	-
DPQ29_RS24395	4720216..4720815	-	600	WP_001314324.1	glucose-1-phosphatase	-
DPQ29_RS24400	4720914..4721699	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T107135 WP_000897302.1 NZ_CP030111:c4716729-4716418 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T107135 NZ_CP030111:c4716729-4716418 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT107135 WP_000356397.1 NZ_CP030111:c4716417-4716127 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT107135 NZ_CP030111:c4716417-4716127 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416