Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3719648..3720342 Replicon chromosome
Accession NZ_CP030111
Organism Escherichia coli strain MCJCHV-1

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag DPQ29_RS19275 Protein ID WP_001263500.1
Coordinates 3719648..3720046 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag DPQ29_RS19280 Protein ID WP_000554758.1
Coordinates 3720049..3720342 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPQ29_RS19250 3715013..3715471 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
DPQ29_RS19255 3715732..3717189 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
DPQ29_RS19260 3717246..3717860 - 615 WP_000602124.1 peptide chain release factor H -
DPQ29_RS19265 3717857..3718996 - 1140 WP_000521561.1 RNA ligase RtcB family protein -
DPQ29_RS19270 3719186..3719638 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
DPQ29_RS19275 3719648..3720046 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
DPQ29_RS19280 3720049..3720342 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
DPQ29_RS19285 3720394..3721439 - 1046 Protein_3532 DNA polymerase IV -
DPQ29_RS19290 3721510..3722433 - 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
DPQ29_RS19295 3722436..3723299 - 864 WP_001169518.1 flagellar motor stator protein MotA -
DPQ29_RS19300 3723312..3724028 - 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
DPQ29_RS19305 3724048..3724515 - 468 WP_000725261.1 flagellar basal body-associated FliL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T107124 WP_001263500.1 NZ_CP030111:c3720046-3719648 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T107124 NZ_CP030111:c3720046-3719648 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT107124 WP_000554758.1 NZ_CP030111:c3720342-3720049 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT107124 NZ_CP030111:c3720342-3720049 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References