Detailed information of TA system TA107124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3719648..3720342
• Replicon: chromosome
• Accession: NZ_CP030111
• Organism: Escherichia coli strain MCJCHV-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: DPQ29_RS19275
• Protein ID: WP_001263500.1
• Coordinates: 3719648..3720046 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: DPQ29_RS19280
• Protein ID: WP_000554758.1
• Coordinates: 3720049..3720342 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPQ29_RS19250	3715013..3715471	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
DPQ29_RS19255	3715732..3717189	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
DPQ29_RS19260	3717246..3717860	-	615	WP_000602124.1	peptide chain release factor H	-
DPQ29_RS19265	3717857..3718996	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
DPQ29_RS19270	3719186..3719638	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
DPQ29_RS19275	3719648..3720046	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DPQ29_RS19280	3720049..3720342	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DPQ29_RS19285	3720394..3721439	-	1046	Protein_3532	DNA polymerase IV	-
DPQ29_RS19290	3721510..3722433	-	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
DPQ29_RS19295	3722436..3723299	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
DPQ29_RS19300	3723312..3724028	-	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
DPQ29_RS19305	3724048..3724515	-	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T107124 WP_001263500.1 NZ_CP030111:c3720046-3719648 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T107124 NZ_CP030111:c3720046-3719648 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT107124 WP_000554758.1 NZ_CP030111:c3720342-3720049 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT107124 NZ_CP030111:c3720342-3720049 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3