Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2450654..2451216 Replicon chromosome
Accession NZ_CP030111
Organism Escherichia coli strain MCJCHV-1

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag DPQ29_RS12525 Protein ID WP_000605675.1
Coordinates 2450654..2450932 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag DPQ29_RS12530 Protein ID WP_000781370.1
Coordinates 2450932..2451216 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPQ29_RS12495 2446240..2446671 - 432 WP_000152305.1 peroxiredoxin OsmC -
DPQ29_RS12505 2447016..2447231 + 216 WP_000495766.1 biofilm-dependent modulation protein -
DPQ29_RS12510 2447333..2447470 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
DPQ29_RS12515 2447627..2449324 + 1698 WP_000433460.1 malate dehydrogenase -
DPQ29_RS12520 2449458..2450468 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
DPQ29_RS12525 2450654..2450932 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DPQ29_RS12530 2450932..2451216 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
DPQ29_RS12535 2451445..2452098 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
DPQ29_RS12540 2452091..2452975 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
DPQ29_RS12545 2452988..2456035 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T107121 WP_000605675.1 NZ_CP030111:2450654-2450932 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T107121 NZ_CP030111:2450654-2450932 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT107121 WP_000781370.1 NZ_CP030111:2450932-2451216 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT107121 NZ_CP030111:2450932-2451216 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References