Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 863406..864076 Replicon chromosome
Accession NZ_CP030093
Organism Mycobacterium tuberculosis strain RUS_B0

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag DPM14_RS04070 Protein ID WP_003899954.1
Coordinates 863732..864076 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag DPM14_RS04065 Protein ID WP_003899955.1
Coordinates 863406..863735 (-) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPM14_RS04035 858821..859855 + 1035 WP_003416786.1 IS30 family transposase -
DPM14_RS04045 860102..860311 - 210 WP_003416778.1 hypothetical protein -
DPM14_RS04050 860479..861744 + 1266 WP_003902423.1 hypothetical protein -
DPM14_RS04055 861904..862524 - 621 WP_003899957.1 RES family NAD+ phosphorylase -
DPM14_RS04060 862521..862868 - 348 WP_003899956.1 DUF2384 domain-containing protein -
DPM14_RS04065 863406..863735 - 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
DPM14_RS04070 863732..864076 - 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DPM14_RS04075 864307..864759 + 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
DPM14_RS04080 864762..865196 + 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
DPM14_RS04085 865305..866566 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
DPM14_RS04090 866901..868190 - 1290 WP_003416640.1 ATP-binding protein -
DPM14_RS04095 868478..868771 - 294 WP_003416635.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T107050 WP_003899954.1 NZ_CP030093:c864076-863732 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T107050 NZ_CP030093:c864076-863732 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT107050 WP_003899955.1 NZ_CP030093:c863735-863406 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT107050 NZ_CP030093:c863735-863406 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References