Detailed information of TA system TA107033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 426249..426759
• Replicon: chromosome
• Accession: NZ_CP030086
• Organism: Polynucleobacter paneuropaeus strain MWH-Creno-4B4

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: DPM16_RS02275
• Protein ID: WP_112203283.1
• Coordinates: 426496..426759 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: DPM16_RS02270
• Protein ID: WP_112203281.1
• Coordinates: 426249..426506 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPM16_RS02235	421359..421802	-	444	WP_112208912.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
DPM16_RS02240	421827..422219	-	393	WP_112203271.1	ribosome silencing factor	-
DPM16_RS02245	422255..422944	-	690	WP_112237701.1	nicotinate (nicotinamide) nucleotide adenylyltransferase	-
DPM16_RS02250	422945..423892	-	948	WP_168183329.1	oxygen-dependent coproporphyrinogen oxidase	-
DPM16_RS02255	423893..425170	-	1278	WP_112237703.1	phosphoribosylamine--glycine ligase	-
DPM16_RS02260	425183..425902	-	720	WP_112237704.1	YebC/PmpR family DNA-binding transcriptional regulator	-
DPM16_RS02270	426249..426506	+	258	WP_112203281.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
DPM16_RS02275	426496..426759	+	264	WP_112203283.1	Txe/YoeB family addiction module toxin	Toxin
DPM16_RS02280	426764..427705	-	942	WP_112203285.1	CysB family HTH-type transcriptional regulator	-
DPM16_RS02285	427769..428923	-	1155	WP_112208915.1	LPS export ABC transporter permease LptG	-
DPM16_RS02290	428920..430035	-	1116	WP_112318117.1	LPS export ABC transporter permease LptF	-
DPM16_RS02295	430146..431723	+	1578	WP_112318118.1	leucyl aminopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10209.77 Da
• Isoelectric Point: 9.4082

>T107033 WP_112203283.1 NZ_CP030086:426496-426759 [Polynucleobacter paneuropaeus]
MRSDLVWTSAAWSDYLYWQGQDKKTLKRINLLIKDAIRNPAEGIGKPEALKENLSGLWSRRIDDVNRLVYVIEKDQLVII
ACRYHYS

Nucleotide

• Length: 264 bp

>T107033 NZ_CP030086:426496-426759 [Polynucleobacter paneuropaeus]
ATGAGAAGTGATTTAGTATGGACCTCTGCTGCTTGGTCCGATTATCTATATTGGCAGGGACAAGATAAAAAAACACTTAA
GCGCATCAATCTTTTGATTAAAGATGCGATACGCAATCCGGCAGAGGGTATCGGTAAGCCCGAGGCTTTGAAAGAAAATC
TATCCGGCTTATGGTCTAGACGCATTGATGATGTAAATCGCTTGGTCTACGTAATCGAGAAAGACCAGTTAGTCATCATT
GCTTGCCGATATCACTACAGTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9638.03 Da
• Isoelectric Point: 8.5493

>AT107033 WP_112203281.1 NZ_CP030086:426249-426506 [Polynucleobacter paneuropaeus]
MRVINFSDARNQFKQVIDQVVADSDVTIISRRDAEDAVVMSLDTYNSMMETVHLLKSPANVKHLEKSLAQYRKGQTKAKA
LVDEK

Nucleotide

• Length: 258 bp

>AT107033 NZ_CP030086:426249-426506 [Polynucleobacter paneuropaeus]
ATGCGCGTCATTAATTTCTCTGATGCTAGAAATCAATTTAAGCAAGTGATCGATCAAGTTGTCGCTGACTCTGATGTAAC
TATTATTTCTCGAAGAGACGCTGAAGATGCTGTGGTGATGTCTTTGGATACCTATAACAGCATGATGGAAACAGTCCATT
TATTAAAATCTCCCGCTAACGTAAAACATCTGGAGAAATCATTGGCGCAATATCGTAAAGGTCAAACCAAAGCCAAGGCT
TTAGTGGATGAGAAGTGA