Detailed information of TA system TA107032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 320866..321457
• Replicon: chromosome
• Accession: NZ_CP030086
• Organism: Polynucleobacter paneuropaeus strain MWH-Creno-4B4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DPM16_RS01760
• Protein ID: WP_112203122.1
• Coordinates: 320866..321144 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DPM16_RS01765
• Protein ID: WP_112205782.1
• Coordinates: 321149..321457 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPM16_RS01735	316113..317141	-	1029	WP_112318076.1	ionic transporter y4hA	-
DPM16_RS01740	317234..317596	+	363	WP_112318077.1	CbiX/SirB N-terminal domain-containing protein	-
DPM16_RS01745	317519..318373	-	855	WP_112318078.1	uroporphyrinogen-III C-methyltransferase	-
DPM16_RS01750	318377..318982	-	606	WP_112318079.1	DUF934 domain-containing protein	-
DPM16_RS01755	318979..320694	-	1716	WP_112318080.1	nitrite/sulfite reductase	-
DPM16_RS01760	320866..321144	+	279	WP_112203122.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DPM16_RS01765	321149..321457	+	309	WP_112205782.1	HigA family addiction module antidote protein	Antitoxin
DPM16_RS01770	321508..321756	+	249	WP_112203124.1	prevent-host-death family protein	-
DPM16_RS01775	321840..322199	-	360	WP_112294892.1	hypothetical protein	-
DPM16_RS01780	322213..322434	-	222	WP_112313704.1	hypothetical protein	-
DPM16_RS01785	322617..323210	+	594	WP_112238541.1	DUF308 domain-containing protein	-
DPM16_RS08205	323253..323423	+	171	WP_158525168.1	hypothetical protein	-
DPM16_RS01790	323515..324582	+	1068	WP_112318081.1	acyltransferase	-
DPM16_RS01795	324587..325114	-	528	WP_112208855.1	DUF615 domain-containing protein	-
DPM16_RS01800	325131..325436	-	306	WP_112205784.1	helix-turn-helix domain-containing protein	-
DPM16_RS01805	325486..325788	-	303	WP_112208856.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-71)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10686.35 Da
• Isoelectric Point: 10.1614

>T107032 WP_112203122.1 NZ_CP030086:320866-321144 [Polynucleobacter paneuropaeus]
MIHSFLCGLTKDLFDSKPSKKFGNIERAARRKLLQLHAATAISDLRTPPGNMLELLIGNRRGQYSIRINHQWRICFVWRE
DGAHNVEIVDYH

Nucleotide

• Length: 279 bp

>T107032 NZ_CP030086:320866-321144 [Polynucleobacter paneuropaeus]
ATGATCCATTCATTCTTATGCGGCCTAACAAAGGACTTATTTGATAGCAAGCCAAGCAAGAAGTTCGGAAATATTGAGAG
GGCGGCAAGACGCAAGTTATTGCAATTGCATGCTGCTACAGCCATATCAGACTTACGAACTCCGCCGGGGAATATGTTGG
AATTGTTGATTGGAAATCGAAGGGGGCAGTACAGCATTCGAATTAATCATCAATGGCGTATATGTTTTGTATGGAGGGAA
GATGGAGCACACAATGTAGAGATTGTGGATTACCACTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11832.82 Da
• Isoelectric Point: 5.7251

>AT107032 WP_112205782.1 NZ_CP030086:321149-321457 [Polynucleobacter paneuropaeus]
MMTTKLLDEIHPGEILLEDFMKPMGITSRQLAADIDVSPSRISEIIHGTRPITADTALRLGLFFSMEPRFWLNLQSEYDM
RMAKRNLQEEIEPRIRVFRIAA

Nucleotide

• Length: 309 bp

>AT107032 NZ_CP030086:321149-321457 [Polynucleobacter paneuropaeus]
ATGATGACAACGAAATTACTTGATGAAATTCATCCTGGCGAAATTTTGCTAGAAGACTTTATGAAGCCAATGGGTATTAC
TTCCCGTCAGCTAGCGGCCGATATTGATGTCTCGCCAAGTCGAATTAGCGAAATCATCCATGGCACTCGCCCCATTACTG
CTGATACAGCATTGCGCTTAGGCTTATTTTTTTCAATGGAGCCCCGCTTTTGGTTGAACTTGCAGTCTGAGTACGACATG
CGAATGGCTAAGAGAAATCTGCAAGAAGAAATAGAACCCAGAATTCGGGTATTTCGAATTGCTGCTTAA