Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3040935..3041592 Replicon chromosome
Accession NZ_CP030076
Organism Enterobacter hormaechei strain 20710

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag DPF84_RS15145 Protein ID WP_015571638.1
Coordinates 3041293..3041592 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A837FB39
Locus tag DPF84_RS15140 Protein ID WP_045346164.1
Coordinates 3040935..3041282 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPF84_RS15115 3036269..3037267 + 999 WP_045346167.1 transketolase family protein -
DPF84_RS15120 3037342..3038073 + 732 WP_022651705.1 class I SAM-dependent methyltransferase -
DPF84_RS15125 3038070..3038672 - 603 WP_022651704.1 short chain dehydrogenase -
DPF84_RS15130 3038770..3039657 + 888 WP_032660046.1 LysR family transcriptional regulator -
DPF84_RS15135 3039678..3040859 + 1182 WP_045346166.1 PLP-dependent aminotransferase family protein -
DPF84_RS15140 3040935..3041282 - 348 WP_045346164.1 HigA family addiction module antidote protein Antitoxin
DPF84_RS15145 3041293..3041592 - 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DPF84_RS15150 3041843..3043003 - 1161 WP_100154277.1 HlyD family efflux transporter periplasmic adaptor subunit -
DPF84_RS15155 3043023..3045215 - 2193 WP_058672812.1 type I secretion system permease/ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3014415..3059932 45517


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T107014 WP_015571638.1 NZ_CP030076:c3041592-3041293 [Enterobacter hormaechei]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

>T107014 NZ_CP030076:c3041592-3041293 [Enterobacter hormaechei]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATACTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13062.08 Da        Isoelectric Point: 9.5932

>AT107014 WP_045346164.1 NZ_CP030076:c3041282-3040935 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKALNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVA

Download         Length: 348 bp

>AT107014 NZ_CP030076:c3041282-3040935 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTAAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCGGACGTTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A837FB39

References