Detailed information of TA system TA107012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1880375..1881105
• Replicon: chromosome
• Accession: NZ_CP030076
• Organism: Enterobacter hormaechei strain 20710

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: DPF84_RS09460
• Protein ID: WP_023302825.1
• Coordinates: 1880791..1881105 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DPF84_RS09455
• Protein ID: WP_023323924.1
• Coordinates: 1880375..1880794 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPF84_RS09430	1875866..1877371	-	1506	WP_045336028.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
DPF84_RS09435	1877368..1877961	-	594	WP_003861054.1	transcriptional regulator UhpA	-
DPF84_RS09440	1878044..1878331	-	288	WP_028014960.1	acetolactate synthase small subunit	-
DPF84_RS09445	1878335..1880023	-	1689	WP_003861056.1	acetolactate synthase large subunit	-
DPF84_RS09450	1880130..1880228	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
DPF84_RS09455	1880375..1880794	-	420	WP_023323924.1	helix-turn-helix domain-containing protein	Antitoxin
DPF84_RS09460	1880791..1881105	-	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
DPF84_RS09465	1881592..1881681	+	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
DPF84_RS09470	1881746..1882192	+	447	WP_003861064.1	GNAT family N-acetyltransferase	-
DPF84_RS09475	1882255..1883088	+	834	WP_045346066.1	DMT family transporter	-
DPF84_RS09480	1883264..1884448	+	1185	WP_003861070.1	multidrug efflux MFS transporter EmrD	-
DPF84_RS09485	1884354..1885439	-	1086	Protein_1795	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Antitoxin HigA (1-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T107012 WP_023302825.1 NZ_CP030076:c1881105-1880791 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T107012 NZ_CP030076:c1881105-1880791 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15499.15 Da
• Isoelectric Point: 5.7670

>AT107012 WP_023323924.1 NZ_CP030076:c1880794-1880375 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISRYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT107012 NZ_CP030076:c1880794-1880375 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTGT
GCGCCAGAATTAGCCGTTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGTTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2