Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1355221..1355818 Replicon chromosome
Accession NZ_CP030072
Organism Klebsiella pneumoniae strain DA12090

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag DL426_RS07485 Protein ID WP_004142563.1
Coordinates 1355221..1355538 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag DL426_RS07490 Protein ID WP_004142561.1
Coordinates 1355531..1355818 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DL426_RS07470 1350924..1352351 - 1428 WP_004176980.1 MFS transporter -
DL426_RS07475 1352460..1353326 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
DL426_RS07480 1353993..1355036 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
DL426_RS07485 1355221..1355538 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DL426_RS07490 1355531..1355818 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
DL426_RS07495 1356083..1356736 - 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
DL426_RS07500 1356856..1357224 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
DL426_RS07505 1357224..1357805 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
DL426_RS07510 1357985..1359100 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
DL426_RS07515 1359131..1359472 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
DL426_RS07520 1359490..1359738 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T106996 WP_004142563.1 NZ_CP030072:1355221-1355538 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T106996 NZ_CP030072:1355221-1355538 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT106996 WP_004142561.1 NZ_CP030072:1355531-1355818 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT106996 NZ_CP030072:1355531-1355818 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References