Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4788943..4789529 Replicon chromosome
Accession NZ_CP030070
Organism Klebsiella pneumoniae strain IA565

Toxin (Protein)


Gene name doc Uniprot ID A0A485R3H6
Locus tag DPF99_RS25650 Protein ID WP_032417643.1
Coordinates 4788943..4789311 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag DPF99_RS25655 Protein ID WP_004174006.1
Coordinates 4789308..4789529 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPF99_RS25630 4784446..4785516 - 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
DPF99_RS25635 4785518..4786363 - 846 WP_032416348.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
DPF99_RS25640 4786360..4787247 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
DPF99_RS25645 4787354..4788670 - 1317 WP_032417642.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
DPF99_RS25650 4788943..4789311 - 369 WP_032417643.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DPF99_RS25655 4789308..4789529 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DPF99_RS25660 4789693..4790406 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
DPF99_RS25665 4790408..4791175 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
DPF99_RS25670 4791172..4792449 - 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
DPF99_RS25675 4792446..4793372 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4783709..4804792 21083


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13584.98 Da        Isoelectric Point: 8.6410

>T106989 WP_032417643.1 NZ_CP030070:c4789311-4788943 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRMLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T106989 NZ_CP030070:c4789311-4788943 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGATGCTAAACAAAATTGAATATGAAGGTGTGACAGATGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT106989 WP_004174006.1 NZ_CP030070:c4789529-4789308 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT106989 NZ_CP030070:c4789529-4789308 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A485R3H6

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References