Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4762694..4763340 Replicon chromosome
Accession NZ_CP030070
Organism Klebsiella pneumoniae strain IA565

Toxin (Protein)


Gene name higB Uniprot ID A0A486UPU4
Locus tag DPF99_RS25540 Protein ID WP_032417639.1
Coordinates 4762993..4763340 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag DPF99_RS25535 Protein ID WP_002920557.1
Coordinates 4762694..4762993 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPF99_RS25515 4758900..4759658 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
DPF99_RS25520 4759708..4760538 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
DPF99_RS25525 4760589..4760918 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
DPF99_RS25530 4761123..4762631 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
DPF99_RS25535 4762694..4762993 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
DPF99_RS25540 4762993..4763340 - 348 WP_032417639.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DPF99_RS25545 4763516..4765963 - 2448 WP_002920561.1 glycogen phosphorylase -
DPF99_RS25550 4765981..4767414 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13527.52 Da        Isoelectric Point: 5.6802

>T106988 WP_032417639.1 NZ_CP030070:c4763340-4762993 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATILILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFEPLRKAI
VLCAGDKDGMNEKRFYKELITLADREFSQHLTKER

Download         Length: 348 bp

>T106988 NZ_CP030070:c4763340-4762993 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATTCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGAGCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGTTGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT106988 WP_002920557.1 NZ_CP030070:c4762993-4762694 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT106988 NZ_CP030070:c4762993-4762694 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCAGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486UPU4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References