Detailed information of TA system TA106946

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): fst-RNAII/Fst(toxin)
• Location: 46470..46712
• Replicon: plasmid pC54
• Accession: NZ_CP030046
• Organism: Enterococcus faecalis strain C54

Toxin (Protein)

• Gene name: fst
• Uniprot ID: -
• Locus tag: DOU32_RS14620
• Protein ID: WP_002360667.1
• Coordinates: 46602..46712 (-)
• Length: 37 a.a.

Antitoxin (RNA)

Gene name	RNAII
Locus tag	-
Coordinates	46470..46561 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DOU32_RS14875	41763..42089	-	327	WP_002387760.1	DNA-binding protein	-
DOU32_RS14585	42126..42290	+	165	WP_001808810.1	helix-turn-helix domain-containing protein	-
DOU32_RS14590	42329..43009	+	681	WP_000191454.1	IS6-like element ISEnfa1 family transposase	-
DOU32_RS14595	43078..43239	-	162	Protein_49	TraB/GumN family protein	-
DOU32_RS14600	43410..44420	-	1011	WP_002360662.1	replication initiator protein A	-
DOU32_RS14605	44680..45036	-	357	WP_128713114.1	hypothetical protein	-
DOU32_RS14610	45029..45811	-	783	WP_002360664.1	ParA family protein	-
DOU32_RS14615	46065..46361	-	297	WP_002360665.1	replication control protein PrgN	-
-	46470..46561	+	92	NuclAT_0	-	Antitoxin
-	46470..46561	+	92	NuclAT_0	-	Antitoxin
-	46470..46561	+	92	NuclAT_0	-	Antitoxin
-	46470..46561	+	92	NuclAT_0	-	Antitoxin
DOU32_RS14620	46602..46712	-	111	WP_002360667.1	type I toxin-antitoxin system Fst family toxin	Toxin
DOU32_RS14630	46963..47313	-	351	WP_002360672.1	hypothetical protein	-
DOU32_RS14635	47310..48638	-	1329	WP_138807346.1	Y-family DNA polymerase	-
DOU32_RS14640	49067..49369	-	303	WP_128713120.1	hypothetical protein	-
DOU32_RS14645	49390..49590	-	201	WP_002390466.1	hypothetical protein	-
DOU32_RS14650	49659..49883	-	225	WP_002358271.1	hypothetical protein	-
DOU32_RS14655	49877..50191	-	315	WP_002390465.1	hypothetical protein	-
DOU32_RS14660	50181..50801	-	621	WP_161978277.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	fexA / optrA / erm(A)	-	1..64500	64500

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Sequences

Toxin

Protein

• Length: 37 a.a.
• Molecular weight: 4117.92 Da
• Isoelectric Point: 4.1672

>T106946 WP_002360667.1 NZ_CP030046:c46712-46602 [Enterococcus faecalis]
VLFVKDLMSLVIAPIFVGLVLEMISRVLDEEDDNRK

Nucleotide

• Length: 111 bp

>T106946 NZ_CP030046:c46712-46602 [Enterococcus faecalis]
GTGTTATTTGTGAAAGATTTAATGTCGTTGGTTATCGCACCGATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGT
GTTGGACGAGGAAGACGATAACCGAAAGTAA

Antitoxin

Nucleotide

• Length: 92 bp

>AT106946 NZ_CP030046:46470-46561 [Enterococcus faecalis]
TAAAAATATGTTATACTAAAGGTGCGAAACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGA
TTGCTTTTTTTT

Structures

Antitoxin