Detailed information of TA system TA106893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 933149..933682
• Replicon: chromosome
• Accession: NZ_CP030039
• Organism: Kocuria sp. BT304

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: DNL27_RS04290
• Protein ID: WP_112234789.1
• Coordinates: 933428..933682 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A1E8UHK7
• Locus tag: DNL27_RS04285
• Protein ID: WP_039101685.1
• Coordinates: 933149..933427 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNL27_RS04250	928164..928523	+	360	WP_112234787.1	metalloregulator ArsR/SmtB family transcription factor	-
DNL27_RS04255	928520..930433	+	1914	WP_204360330.1	cadmium-translocating P-type ATPase	-
DNL27_RS04260	930430..930738	+	309	WP_012397801.1	hypothetical protein	-
DNL27_RS04265	930963..931514	+	552	WP_012397802.1	hypothetical protein	-
DNL27_RS04270	931536..932306	-	771	Protein_833	hypothetical protein	-
DNL27_RS04275	932342..932638	-	297	Protein_834	IS3 family transposase	-
DNL27_RS04280	932676..932984	+	309	WP_039101684.1	HigA family addiction module antidote protein	-
DNL27_RS04285	933149..933427	+	279	WP_039101685.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
DNL27_RS04290	933428..933682	+	255	WP_112234789.1	Txe/YoeB family addiction module toxin	Toxin
DNL27_RS04300	934091..935521	+	1431	WP_112234790.1	anion permease	-
DNL27_RS04305	935610..936359	-	750	WP_112234791.1	succinate dehydrogenase/fumarate reductase iron-sulfur subunit	-
DNL27_RS04310	936373..938361	-	1989	WP_112234792.1	fumarate reductase/succinate dehydrogenase flavoprotein subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-67)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10254.73 Da
• Isoelectric Point: 8.0094

>T106893 WP_112234789.1 NZ_CP030039:933428-933682 [Kocuria sp. BT304]
MRLVWDEAAWEDYTYWQTADRRILKRINLLIDACLREPFERIGKPEQLKYGARGSWSRRINEEHRLVYLVTGEDLVILQA
RYHY

Nucleotide

• Length: 255 bp

>T106893 NZ_CP030039:933428-933682 [Kocuria sp. BT304]
ATGCGGCTGGTCTGGGACGAGGCCGCCTGGGAGGACTACACGTACTGGCAGACCGCCGACCGGAGAATCCTCAAGCGGAT
CAACCTGCTCATCGATGCCTGCCTGCGCGAGCCGTTCGAGCGCATCGGCAAGCCGGAGCAGTTGAAGTACGGGGCGCGGG
GCTCGTGGTCGCGGCGCATCAACGAGGAGCACCGGCTCGTCTACCTCGTGACTGGTGAGGATCTCGTGATCTTGCAGGCT
CGATACCACTACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10450.67 Da
• Isoelectric Point: 4.8723

>AT106893 WP_039101685.1 NZ_CP030039:933149-933427 [Kocuria sp. BT304]
MPISASEARKTLFPLIERVNEDQEAIEIVSRKGNAVLMPADEYAAWQETAYLFRSPSNARRLLDAYDRARVGKTQVHELD
RSDESVSQARDV

Nucleotide

• Length: 279 bp

>AT106893 NZ_CP030039:933149-933427 [Kocuria sp. BT304]
ATGCCCATCAGCGCAAGCGAGGCCCGCAAGACTCTCTTCCCGCTCATCGAGCGCGTGAACGAGGACCAAGAAGCCATCGA
GATCGTCTCTCGGAAGGGCAACGCGGTGCTCATGCCCGCCGACGAGTACGCGGCGTGGCAGGAGACCGCCTACCTGTTCC
GGTCGCCGTCGAACGCCCGCAGGCTTCTCGACGCCTACGATCGTGCCCGTGTCGGAAAGACCCAGGTCCACGAGCTCGAC
CGTTCCGACGAGTCGGTCAGCCAGGCTCGGGACGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1E8UHK7