Detailed information of TA system TA106888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2991128..2991730
• Replicon: chromosome
• Accession: NZ_CP030035
• Organism: Bacteriovorax stolpii strain AC01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DOM21_RS15050
• Protein ID: WP_102242608.1
• Coordinates: 2991128..2991421 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DOM21_RS15055
• Protein ID: WP_102242607.1
• Coordinates: 2991431..2991730 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DOM21_RS15025	2986515..2987234	+	720	WP_142410003.1	cytochrome b/b6 domain-containing protein	-
DOM21_RS15030	2987244..2987750	+	507	WP_102242612.1	helix-turn-helix transcriptional regulator	-
DOM21_RS15035	2987755..2988444	-	690	WP_142410004.1	hypothetical protein	-
DOM21_RS15040	2988447..2989028	-	582	WP_142410005.1	GNAT family N-acetyltransferase	-
DOM21_RS15045	2989179..2990993	+	1815	WP_102242609.1	M3 family oligoendopeptidase	-
DOM21_RS15050	2991128..2991421	+	294	WP_102242608.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DOM21_RS15055	2991431..2991730	+	300	WP_102242607.1	HigA family addiction module antidote protein	Antitoxin
DOM21_RS15060	2991727..2993034	-	1308	WP_102242606.1	MBL fold metallo-hydrolase	-
DOM21_RS15065	2993044..2993889	-	846	WP_142410006.1	restriction endonuclease	-
DOM21_RS15070	2993999..2994727	+	729	WP_142410007.1	SDR family oxidoreductase	-
DOM21_RS15075	2994828..2995253	+	426	WP_142410008.1	hypothetical protein	-
DOM21_RS15080	2995392..2995667	+	276	WP_142410316.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
DOM21_RS15085	2995657..2995923	+	267	WP_102242602.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2502027..3048886	546859

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-96)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (7-92)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11400.32 Da
• Isoelectric Point: 8.9433

>T106888 WP_102242608.1 NZ_CP030035:2991128-2991421 [Bacteriovorax stolpii]
MVIVSFREIQLQDFIETGIPPKNCQWKFLSKVVLRKLDMLNYAKRLDDLKAPPGNRLKALKGDLIGHYSIRINDQWRIIF
IWSEDGPANVEIVDYHF

Nucleotide

• Length: 294 bp

>T106888 NZ_CP030035:2991128-2991421 [Bacteriovorax stolpii]
ATGGTTATTGTCAGTTTCAGGGAGATTCAACTTCAAGATTTTATAGAAACGGGAATTCCTCCGAAGAATTGCCAATGGAA
ATTTTTATCTAAAGTAGTTTTAAGAAAGCTGGATATGTTAAACTACGCAAAACGGTTGGATGATTTAAAAGCTCCACCTG
GAAACAGGCTCAAAGCTCTTAAAGGTGATTTAATCGGTCATTACAGTATAAGAATTAACGACCAGTGGAGAATTATTTTT
ATCTGGAGTGAAGATGGTCCAGCGAATGTTGAAATAGTTGATTACCACTTTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11157.98 Da
• Isoelectric Point: 9.4884

>AT106888 WP_102242607.1 NZ_CP030035:2991431-2991730 [Bacteriovorax stolpii]
MNKINYQRPPTNPGEILSEEFLKPLGLTQKELADHIQVDIKVINRLVNGKTTLSAEMALKLASSFQTTPDFWLSAQYEID
LYRAKMSLPKLPGAIIKTN

Nucleotide

• Length: 300 bp

>AT106888 NZ_CP030035:2991431-2991730 [Bacteriovorax stolpii]
ATGAATAAAATAAACTATCAAAGACCACCGACGAATCCAGGAGAAATTCTTTCCGAAGAATTTTTAAAACCTTTGGGATT
AACTCAGAAAGAATTGGCCGACCATATACAAGTTGATATCAAAGTGATTAATCGACTGGTAAATGGAAAAACGACCTTAT
CTGCCGAAATGGCCTTAAAACTCGCTTCTTCTTTTCAAACGACGCCAGACTTCTGGCTATCGGCCCAATACGAAATTGAT
CTTTATAGAGCAAAAATGTCTCTTCCAAAACTTCCCGGCGCAATTATTAAGACTAATTAA