Detailed information of TA system TA106823

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 7454..7871
• Replicon: plasmid unnamed3
• Accession: NZ_CP029982
• Organism: Escherichia coli strain 99-3165

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: B4O06_RS25890
• Protein ID: WP_001312861.1
• Coordinates: 7713..7871 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	7454..7648 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4O06_RS25850	2579..3147	+	569	Protein_2	transposase	-
B4O06_RS26700	3334..3477	-	144	Protein_3	IS91 family transposase	-
B4O06_RS25865	3462..3827	-	366	WP_000124102.1	hypothetical protein	-
B4O06_RS25870	4104..4940	-	837	Protein_5	IS91 family transposase	-
B4O06_RS25875	4939..6237	+	1299	Protein_6	hypothetical protein	-
B4O06_RS25880	6292..6726	+	435	WP_000845924.1	conjugation system SOS inhibitor PsiB	-
B4O06_RS25885	6723..7442	+	720	WP_001276221.1	plasmid SOS inhibition protein A	-
-	7454..7648	+	195	NuclAT_0	-	Antitoxin
-	7454..7648	+	195	NuclAT_0	-	Antitoxin
-	7454..7648	+	195	NuclAT_0	-	Antitoxin
-	7454..7648	+	195	NuclAT_0	-	Antitoxin
B4O06_RS25890	7713..7871	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
B4O06_RS25895	8296..9512	+	1217	Protein_10	IS3 family transposase	-
B4O06_RS25900	10043..11210	+	1168	Protein_11	plasmid-partitioning protein SopA	-
B4O06_RS25905	11227..12181	+	955	Protein_12	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..71543	71543
	-	inside	IScluster/Tn	-	-	1513..18057	16544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T106823 WP_001312861.1 NZ_CP029982:7713-7871 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T106823 NZ_CP029982:7713-7871 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 195 bp

>AT106823 NZ_CP029982:7454-7648 [Escherichia coli]
TCACGCGGATTTCCCGTAGCCTGAATGAGCGTATTCTTTTCAGGAAAAGTGAATGTGGCCAGCGCGCAGGGATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACAGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin