Detailed information of TA system TA106821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3930784..3931478
• Replicon: chromosome
• Accession: NZ_CP029981
• Organism: Escherichia coli strain 99-3165

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: B4O06_RS21770
• Protein ID: WP_001263493.1
• Coordinates: 3930784..3931182 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: B4O06_RS21775
• Protein ID: WP_000554757.1
• Coordinates: 3931185..3931478 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4O06_RS21740	3925784..3927028	-	1245	WP_000189541.1	esterase FrsA	-
-	3926444..3926524	-	81	NuclAT_12	-	-
-	3926444..3926524	-	81	NuclAT_12	-	-
-	3926444..3926524	-	81	NuclAT_12	-	-
-	3926444..3926524	-	81	NuclAT_12	-	-
B4O06_RS21745	3927120..3927578	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
B4O06_RS21750	3927839..3929296	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
B4O06_RS21755	3929353..3929874	-	522	Protein_3759	peptide chain release factor H	-
B4O06_RS21760	3929873..3930076	-	204	Protein_3760	RNA ligase RtcB family protein	-
B4O06_RS21765	3930322..3930774	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
B4O06_RS21770	3930784..3931182	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
B4O06_RS21775	3931185..3931478	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
B4O06_RS21780	3931530..3932585	-	1056	WP_001226164.1	DNA polymerase IV	-
B4O06_RS21785	3932656..3933579	-	924	WP_001232570.1	putative lateral flagellar export/assembly protein LafU	-
B4O06_RS21790	3933582..3934445	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
B4O06_RS21795	3934458..3935177	-	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
B4O06_RS21800	3935197..3935664	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T106821 WP_001263493.1 NZ_CP029981:c3931182-3930784 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T106821 NZ_CP029981:c3931182-3930784 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT106821 WP_000554757.1 NZ_CP029981:c3931478-3931185 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT106821 NZ_CP029981:c3931478-3931185 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6