Detailed information of TA system TA106796

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35294..35548
• Replicon: plasmid p51008369SK1_E
• Accession: NZ_CP029978
• Organism: Escherichia coli strain 51008369SK1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: DNQ41_RS27040
• Protein ID: WP_015387399.1
• Coordinates: 35342..35548 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	35294..35355 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNQ41_RS26990	30946..31158	+	213	WP_005012601.1	hypothetical protein	-
DNQ41_RS28000	31459..31548	-	90	Protein_34	IS1 family transposase	-
DNQ41_RS27005	31603..32280	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
DNQ41_RS27010	32280..32627	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
DNQ41_RS27015	32647..34218	+	1572	WP_023149734.1	IS66 family transposase	-
DNQ41_RS27020	34256..34872	-	617	Protein_38	IS1 family transposase	-
DNQ41_RS27030	34973..35155	+	183	WP_000968309.1	hypothetical protein	-
-	35294..35355	-	62	NuclAT_0	-	Antitoxin
-	35294..35355	-	62	NuclAT_0	-	Antitoxin
-	35294..35355	-	62	NuclAT_0	-	Antitoxin
-	35294..35355	-	62	NuclAT_0	-	Antitoxin
DNQ41_RS27040	35342..35548	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
DNQ41_RS27045	35832..36089	+	258	WP_000083833.1	replication regulatory protein RepA	-
DNQ41_RS27055	36325..36399	+	75	WP_032336874.1	RepA leader peptide Tap	-
DNQ41_RS27060	36392..36838	+	447	Protein_43	incFII family plasmid replication initiator RepA	-
DNQ41_RS27065	36784..37266	-	483	Protein_44	VENN motif pre-toxin domain-containing protein	-
DNQ41_RS27070	38159..39379	+	1221	WP_000410951.1	arginine deiminase	-
DNQ41_RS27075	39390..40301	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / aac(3)-IIa / mph(A)	iucA / iucB / iucC / iucD / iutA	1..99465	99465

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T106796 WP_015387399.1 NZ_CP029978:35342-35548 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T106796 NZ_CP029978:35342-35548 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT106796 NZ_CP029978:c35355-35294 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin