Detailed information of TA system TA106786

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25730..25994
• Replicon: plasmid p51008369SK1_B
• Accession: NZ_CP029975
• Organism: Escherichia coli strain 51008369SK1

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: DNQ41_RS25460
• Protein ID: WP_001303307.1
• Coordinates: 25730..25882 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	25932..25994 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DNQ41_RS25435	21575..22237	+	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
DNQ41_RS25440	22335..22544	+	210	WP_032178830.1	hemolysin expression modulator Hha	-
DNQ41_RS25445	22876..23280	-	405	WP_001175009.1	IS200/IS605 family transposase	-
DNQ41_RS25450	23339..24565	+	1227	WP_024186316.1	transposase	-
DNQ41_RS27925	24610..24747	+	138	WP_047088497.1	hypothetical protein	-
DNQ41_RS25455	25407..25658	+	252	WP_001291965.1	hypothetical protein	-
DNQ41_RS25460	25730..25882	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	25932..25994	+	63	NuclAT_0	-	Antitoxin
-	25932..25994	+	63	NuclAT_0	-	Antitoxin
-	25932..25994	+	63	NuclAT_0	-	Antitoxin
-	25932..25994	+	63	NuclAT_0	-	Antitoxin
DNQ41_RS25465	26174..27382	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
DNQ41_RS25470	27401..28471	+	1071	WP_000151576.1	IncI1-type conjugal transfer protein TrbB	-
DNQ41_RS25475	28464..30755	+	2292	WP_001289275.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA1 / ant(3'')-Ia / qacE / sul1 / blaTEM-30 / floR / sul2	-	1..113340	113340

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T106786 WP_001303307.1 NZ_CP029975:c25882-25730 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T106786 NZ_CP029975:c25882-25730 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT106786 NZ_CP029975:25932-25994 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin